Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease

Comino, I, Real, A, de Lorenzo, L, Cornell, H, Angel Lopez-Casado, M, Barro, F, Lorite, P, Isabel Torres, M, Cebolla, A and Sousa, C 2011, 'Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease', Gut, vol. 60, no. 7, pp. 915-922.


Document type: Journal Article
Collection: Journal Articles

Title Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
Author(s) Comino, I
Real, A
de Lorenzo, L
Cornell, H
Angel Lopez-Casado, M
Barro, F
Lorite, P
Isabel Torres, M
Cebolla, A
Sousa, C
Year 2011
Journal name Gut
Volume number 60
Issue number 7
Start page 915
End page 922
Total pages 8
Publisher B M J Group
Abstract Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patients with CD. Monoclonal antibodies (moAbs) against the main immunotoxic 33-mer peptide (A1 and G12) react strongly against wheat, barley and rye but have less reactivity against oats. The stated aim of this study is to test whether this observed reactivity could be related to the potential toxicity of oats for patients with CD. Methods In the present study, different oat varieties, controlled for their purity and by their distinct protein pattern, were used to examine differences in moAb G12 recognition by ELISA and western blot. Immunogenicity of oat varieties was determined by 33-mer concentration, T cell proliferation and interferon g production. Results Three groups of oat cultivars reacting differently against moAb G12 could be distinguished: a group with considerable affinity, a group showing slight reactivity and a third with no detectable reactivity. The immunogenicity of the three types of oats as well as that of a positive and negative control was determined with isolated peripheral blood mononuclear T cells from patients with CD by measurement of cell proliferation and interferon g release. A direct correlation of the reactivity with G12 and the immunogenicity of the different prolamins was observed. Conclusions The results showed that the reactivity of the moAb G12 is proportional to the potential immunotoxicity of the cereal cultivar. These differences may explain the different clinical responses observed in patients suffering from CD and open up a means to identify immunologically safe oat cultivars, which could be used to enrich a gluten-free diet.
Subject Allergy
DOI - identifier 10.1136/gut.2010.225268
Copyright notice ©
ISSN 0017-5749
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 95 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 83 times in Scopus Article | Citations
Altmetric details:
Access Statistics: 209 Abstract Views  -  Detailed Statistics
Created: Fri, 07 Oct 2011, 08:50:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us