Cultivation and quality studies of Danshen (Salvia miltiorrhiza) in Australia

Sheng, S 2007, Cultivation and quality studies of Danshen (Salvia miltiorrhiza) in Australia, Doctor of Philosophy (PhD), Health Sciences, RMIT University.


Document type: Thesis
Collection: Theses

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Title Cultivation and quality studies of Danshen (Salvia miltiorrhiza) in Australia
Author(s) Sheng, S
Year 2007
Abstract Danshen (Salvia miltiorrhiza Bunge) is a commonly used Chinese medicinal herb for treating cardiovascular and cerebrovascular diseases. This thesis aims to determine which Danshen varieties are most suitable for local cultivation in Australia, including the optimum harvesting times, growth densities and fertiliser conditions. A two-year field trial was conducted. Among the eight varieties (V1-V8), significant variations were found in root yields and contents of the bioactive marker compounds. The contents of tanshinone IIA and salvianolic acid B in V1, V2, V3 and V6 reached the standards as set in the Chinese Pharmacopoeia. In particular, V6 was the best variety for tanshinones, and V4 and V5 were best varieties for salvianolic acid B. V4 showed a great variation in the growth of roots and aerial parts, and the quantities of the four marker compounds and other main chemical components. The optimum harvesting time for V4 was at 250 days for the roots with the best root yield and the highest salvianolic acid B content, and 290 days for the roots with the best root yield and the highest tanshinone IIA content. Moreover, V4 roots showed a higher content of tanshinones in the first year cultivation, but a higher content of salvianolic acid B in the second year cultivation. V4, grown at 30 × 30 cm, produced the highest root yield per square meter and the highest content of the three tanshinones (e.g. 0.210% tanshinone IIA, w/w); in contrast, when grown at 45 × 30 cm, it produced a high salvianolic acid B content (>3%) and the highest root yield per square meter in three densities (30 × 40 cm, 45 × 30 cm and 45 × 40 cm). Different fertiliser treatments significantly increased the root yield of V1. In particular, a high level phosphorus and nitrogen showed a synergistic effect on root yield. However, an optimum fertiliser treatment for maximum root yield and content of tanshinones was not determined. Both qualitative and quantitative analysis, using marker compounds and chromatographic fingerprint, by TLC and/or HPLC, were developed for assessing the quality of Australian grown Danshen. The findings showed that the three tanshinone markers or their corresponding peaks correlated linearly with each other as well as with other main peaks in chromatographic fingerprints of methanol extracts. Similarly, salvianolic acid B or its corresponding peak correlated linearly with the other main peaks in chromatographic fingerprints of aqueous extracts. In conclusion, the present findings indicate that under proper conditions of harvesting time, fertilisation and plant density, high quality Danshen varieties can be cultivated in Victoria, Australia.
Degree Doctor of Philosophy (PhD)
Institution RMIT University
School, Department or Centre Health Sciences
Keyword(s) Danshen
Salvia miltiorrhiza Bunge
Radix Salviae Miltiorrhiza
cultivation
quality studies in Australia
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