Surface acoustic waves (SAW) mediated crumpled polyethyleneimine-labelled graphene oxide (CGO-PEI)synthesis as nanotherapeutic delivery platform

Yasmine Abbas, Y 2018, Surface acoustic waves (SAW) mediated crumpled polyethyleneimine-labelled graphene oxide (CGO-PEI)synthesis as nanotherapeutic delivery platform, Masters by Research, Engineering, RMIT University.


Document type: Thesis
Collection: Theses

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Title Surface acoustic waves (SAW) mediated crumpled polyethyleneimine-labelled graphene oxide (CGO-PEI)synthesis as nanotherapeutic delivery platform
Author(s) Yasmine Abbas, Y
Year 2018
Abstract Graphene oxide (GO) has been attracting tremendous attention in the past few years in various biomedical applications including; tissue engineering, cell culture, biosensing, bioimaging and therapeutics delivery due to unique features it possesses. In this thesis, we demonstrate the preparation of crumpled GO functionalized with polyethyleneimine (PEI) (CGO-PEI) via surface acoustic waves (SAW) to develop a nanotherapeutic delivery platform with efficient cellular uptake and good biocompatibility. Using the SAW, CGO and CGO-PEI of nano-sized range (307.5±11.8 nm and 281.9±23.3 nm; respectively) were obtained. Functionalization of GO with PEI was successful as indicated by Fourier-transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA) showing that PEI represents 23% of the total weight. After functionalization and nebulization, GO maintained its characteristics as provided by Raman. Finally, we found that functionalizing GO with PEI and crumpling it, have reduced the cytotoxicity accompanying PEI more than GO-PEI sheets from ~18% and ~70% to ~97% in comparison to PEI and GO-PEI sheets; respectively. As well as the PEI-functionalization has enhanced GO’s cellular uptake by about 1.5 times. These results indicate that SAW have the potential to provide CGO-PEI as a nanotherapeutic delivery platform encapsulating biological and delicate therapeutics for treatment of various diseases such as cancer and genetic disorders in the future.
Degree Masters by Research
Institution RMIT University
School, Department or Centre Engineering
Subjects Nanomaterials
Keyword(s) Crumpling
Graphene oxide
Polyethyleneimine
Surface Acoustic Waves (SAW)
Nanotherapeutic Delivery Platform
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Created: Wed, 08 Aug 2018, 15:01:46 EST by Adam Rivett
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