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Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish

Harford, A, O'Halloran, K and Wright, P 2006, 'Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish', Fish and Shellfish Immunology, vol. 20, pp. 562-573.

Document type: Journal Article
Collection: Journal Articles

Title Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish
Author(s) Harford, A
O'Halloran, K
Wright, P
Year 2006
Journal name Fish and Shellfish Immunology
Volume number 20
Start page 562
End page 573
Total pages 11
Publisher Academic Press
Abstract The phagocytic activity of fish immunocytes has been measured by a wide range of methods, and has been used as a bio-indicator to assess the immunotoxicity of environmental pollutants and the efficiency of immunostimulants used in aquaculture. This study demonstrates the utilisation of a flow cytometric technique for measuring phagocytosis as an alternative to manual evaluations by light microscopy. Optimal conditions for the phagocytosis of latex beads were ascertained, including incubation period, cell:bead ratio and media components, for head kidney cells isolated from three native Australian fish that inhabit the Murray-Darling basin, i.e. silver perch (Bidyanus bidyanus), golden perch (Macquaria ambigua) and crimson-spotted rainbowfish (Melanotaenia fluviatilis). Thus, standardised protocols have now been established for future use in the immunotoxicity testing of xenobiotics in native Australian freshwater fish.
Subject Veterinary Immunology
Copyright notice Copyright © 2006 Elsevier Ltd All rights reserved.
ISSN 1050-4648
 
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