Inter-simple sequence repeat markers reveal high genetic diversity among alternaria alternata isolates of Indian origin

Kale, S, Pardeshi, V, Gurjar, G, Gupta, V, Gohokar, R, Ghorpade, P and Kadoo, N 2012, 'Inter-simple sequence repeat markers reveal high genetic diversity among alternaria alternata isolates of Indian origin', Journal of Mycology and Plant Pathology, vol. 42, no. 2, pp. 194-200.


Document type: Journal Article
Collection: Journal Articles

Title Inter-simple sequence repeat markers reveal high genetic diversity among alternaria alternata isolates of Indian origin
Author(s) Kale, S
Pardeshi, V
Gurjar, G
Gupta, V
Gohokar, R
Ghorpade, P
Kadoo, N
Year 2012
Journal name Journal of Mycology and Plant Pathology
Volume number 42
Issue number 2
Start page 194
End page 200
Total pages 7
Publisher Scientific Publishers
Abstract Alternaria spp. is an important pathogen of linseed (Linum usitatissimum L.) causing blight disease, which results in up to 90% yield losses. Genetic relationships among 20 Alternaria alternata isolates, collected from 14 sites located in two states of India, were analyzed using ISSR markers. Eighty-seven loci were amplified using nine primers, of which 86 (98%) were polymorphic. The average number of amplified and polymorphic loci per primer was 9.6 and 9.5 respectively. The range of polymorphism information content (PIC) scores per primer was 0.09 - 0.50, with an average of 0.35. A high level of genetic differentiation among the isolates was detected based on Nei's genetic diversity analysis (0.32) and Shannon's diversity index (0.48). The Jaccard's similarity coefficient among the isolates ranged from 0.14 to 0.87. A dendrogram was generated using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), wherein twelve Alternaria isolates were grouped in two clusters, while eight remained un-clustered. The cluster 1 included isolates from south India as well as Nagpur area, while cluster 2 included isolates from Vidarbha region of Maharashtra. Interestingly, the Mehkar, Kalamb and Amravati isolates from Vidarbha did not group with either of these clusters indicating diverse nature of these isolates. The present study revealed high genetic diversity among the 20 isolates and found that the ISSR markers were useful for the analysis.
Subject Molecular Evolution
Keyword(s) Inter-simple sequence repeat markers reveal high genetic diversity among Alternaria alternata isolates of Indian origin
alternaria
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