Caricain: a basis for enzyme therapy for coeliac disease

Cornell, H and Stelmasiak, T 2011, 'Caricain: a basis for enzyme therapy for coeliac disease ', South African Journal Of Science , vol. 107, no. 910, pp. 74-78.


Document type: Journal Article
Collection: Journal Articles

Title Caricain: a basis for enzyme therapy for coeliac disease
Author(s) Cornell, H
Stelmasiak, T
Year 2011
Journal name South African Journal Of Science
Volume number 107
Issue number 910
Start page 74
End page 78
Total pages 5
Publisher A O S I S Pty. Ltd.
Abstract Gliadin, a glycoprotein present in wheat and other grass cereals, is a causative agent in coeliac disease. It is therefore important to find methods for the detoxification of gliadin. Lysosomal integrity is lost in patients with active coeliac disease but restored when gliadin is removed from the diet. We employed a rat liver lysosome assay to monitor the extent of detoxification of a gliadin digest by caricain, a protein enzyme found in papaya. Pre-incubating the gliadin digest for different durations with caricain allowed the kinetics of the detoxification process to be studied. A significant degree of protection (80%) of the lysosomes was achieved with 1.7% w/w of caricain on substrate after incubation for 2 h at 37 °C. The detoxification followed first-order kinetics with a rate constant of 1.7 × 10 -4/s. The enzyme was strongly inhibited by imidazole, but weakly by phenylmethyl sulphonyl fluoride, as was also a caricain-enriched fraction from ion-exchange chromatography of papaya oleo-resin. The value of caricain in the detoxification of gliadin was confirmed in the present studies and this enzyme shows promise for enzyme therapy in coeliac disease.
Keyword(s) bioassay
biotechnology
cytology
detoxification
diet
digestive system disorder
disease treatment
enzyme activity
fruit
immune system
ion chromatography
phenolic compound
protein
reaction kinetics
resin
rodent
substrate
DOI - identifier 10.4102/sajs.v107i9/10.529
Copyright notice © 2011. The Authors.
ISSN 0038-2353
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