Molecular assays reveal the presence and diversity of genes encoding pea footrot pathogenicity determinants in nectria haematococca and in agricultural soils

Etebu, E and Osborn, A 2009, 'Molecular assays reveal the presence and diversity of genes encoding pea footrot pathogenicity determinants in nectria haematococca and in agricultural soils', Journal of Applied Microbiology, vol. 106, no. 5, pp. 1629-1639.


Document type: Journal Article
Collection: Journal Articles

Title Molecular assays reveal the presence and diversity of genes encoding pea footrot pathogenicity determinants in nectria haematococca and in agricultural soils
Author(s) Etebu, E
Osborn, A
Year 2009
Journal name Journal of Applied Microbiology
Volume number 106
Issue number 5
Start page 1629
End page 1639
Total pages 11
Publisher Wiley-Blackwell Publishing
Abstract Aim: The aim of this study was to develop molecular assays for investigating the presence and diversity of pathogenicity genes from the pea footrot pathogen Nectria haematococca (anamorph Fusarium solani f.sp. pisi) in soils. Methods and Results: Polymerase chain reaction (PCR) assays were developed to amplify four N. haematococca pathogenicity genes (PDA, PEP1, PEP3 and PEP5) from isolates and soil-DNA from five agricultural fields with a prior footrot history. A collection of 15 fungi isolated on medium selective for Fusarium spp. exhibited variation in their virulence to peas as assessed via a disease index (DI: 0-5; no virulence to the highest virulence). PCR analyses showed that three isolates in which all four pathogenicity genes were detected resulted in the highest DI (>3·88). All four pathogenicity genes were detected in soil-DNA obtained from all five fields with a footrot disease history, but were not amplified from soils, which had no footrot history. Denaturing gradient gel electrophoresis and/or sequence analysis revealed diversity amongst the pathogenicity genes. Conclusion: The PCR assays developed herein enable the specific detection of pathogenic N. haematococca in soils without recourse to culture. Significance and Impact of the Study: Molecular assays that specifically target pathogenicity genes have the capacity to assess the presence of the footrot-causing pathogen in agricultural soils.
Subject Microbiology not elsewhere classified
Biological Sciences not elsewhere classified
Keyword(s) Footrot disease in peas
Fusarium solani
Nectria haematococca
Pathogenicity genes (PEP
PDA)
Soil DNA
DOI - identifier 10.1111/j.1365-2672.2008.04130.x
Copyright notice © 2009 The Society for Applied Microbiology
ISSN 1364-5072
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