A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems

Deines, P, Sekar, R, Husband, P, Boxall, J, Osborn, A and Biggs, C 2010, 'A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems', Applied Microbiology and Biotechnology, vol. 87, no. 2, pp. 749-756.


Document type: Journal Article
Collection: Journal Articles

Title A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems
Author(s) Deines, P
Sekar, R
Husband, P
Boxall, J
Osborn, A
Biggs, C
Year 2010
Journal name Applied Microbiology and Biotechnology
Volume number 87
Issue number 2
Start page 749
End page 756
Total pages 8
Publisher Springer
Abstract This study presents a new coupon sampling device that can be inserted directly into the pipes within water distribution systems (WDS), maintaining representative near wall pipe flow conditions and enabling simultaneous microscopy and DNA-based analysis of biofilms formed in situ. To evaluate this sampling device, fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) analyses were used to investigate changes in biofilms on replicate coupons within a non-sterile pilot-scale WDS. FISH analysis demonstrated increases in bacterial biofilm coverage of the coupon surface over time, while the DGGE analysis showed the development of increasingly complex biofilm communities, with time-specific clustering of these communities. This coupon design offers improvements over existing biofilm sampling devices in that it enables simultaneous quantitative and qualitative compositional characterization of biofilm assemblages formed within a WDS, while importantly maintaining fully representative near wall pipe flow conditions. Hence, it provides a practical approach that can be used to capture the interactions between biofilm formation and changing abiotic conditions, boundary shear stress, and turbulent driven exchange within WDS.
Subject Biological Sciences not elsewhere classified
Keyword(s) Biofilms
CARD-FISH
DGGE
Drinking water distribution systems
Sampling coupon
DOI - identifier 10.1007/s00253-010-2510-x
Copyright notice © Springer-Verlag 2010
ISSN 0175-7598
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Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
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