Characterisation of local immune responses induced by a novel nano-particle based carrier-adjuvant in sheep

Gamvrellis, A, Gloster, S, Jefferies, M, Mottram, P, Smooker, P, Plebanski, M and Scheerlinck, J 2013, 'Characterisation of local immune responses induced by a novel nano-particle based carrier-adjuvant in sheep', Veterinary Immunology and Immunopathology, vol. 155, no. 1-2, pp. 21-29.


Document type: Journal Article
Collection: Journal Articles

Title Characterisation of local immune responses induced by a novel nano-particle based carrier-adjuvant in sheep
Author(s) Gamvrellis, A
Gloster, S
Jefferies, M
Mottram, P
Smooker, P
Plebanski, M
Scheerlinck, J
Year 2013
Journal name Veterinary Immunology and Immunopathology
Volume number 155
Issue number 1-2
Start page 21
End page 29
Total pages 9
Publisher Elsevier B.V.
Abstract Most adjuvants require danger signals to promote immune responses against vaccine antigens. Our previous studies have characterised a powerful nano-particulate antigen delivery system, which by itself does not induce inflammation, and which further appears to induce substantial immune responses in mice and sheep without the requirement for added stimulators of toll like receptors or other pathogen recognition receptors. In the present study we dissect the nature of the early induction phase of the immune response stimulated by such a vaccine comprising 40 nm polystyrene nano-particles conjugated to the antigen. We analyse the kinetics of export from an individual draining lymph node from the sheep, of antibodies and cytokines as well as antigen responsive CD4 and CD8 T cells. Our results indicate that simple inert nano-bead based antigen delivery into the draining area of the lymph node is highly efficient at priming combined humoral and T cell antigen specific immunity without the need for added 'danger signals'. Furthermore this nano-bead adjuvant is a potent agent capable of promoting cross-priming for CD8 T cell induction in sheep. Interestingly, using nano-beads, similarly to what has been observed with natural pathogen based lymph node stimulation, a phase of CD4 T cell priming and export preceded CD8 T cell induction, suggesting the engagement of natural priming processes and kinetics.
Subject Biological Sciences not elsewhere classified
Keyword(s) Adjuvants
Nano-beads
Ovine
Vaccines
DOI - identifier 10.1016/j.vetimm.2013.05.015
Copyright notice © 2013 Elsevier B.V. All rights reserved.
ISSN 0165-2427
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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