Differentiation between Campylobacter hyoilei and Campylobater coli using genotypic and phenotypic analyses

Dep, M, Mendz, G, Trend, M, Coloe, P, Korolik, V and Fry, B 2001, 'Differentiation between Campylobacter hyoilei and Campylobater coli using genotypic and phenotypic analyses', International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 3, pp. 819-826.


Document type: Journal Article
Collection: Journal Articles

Title Differentiation between Campylobacter hyoilei and Campylobater coli using genotypic and phenotypic analyses
Author(s) Dep, M
Mendz, G
Trend, M
Coloe, P
Korolik, V
Fry, B
Year 2001
Journal name International Journal of Systematic and Evolutionary Microbiology
Volume number 51
Issue number 3
Start page 819
End page 826
Total pages 7
Publisher Society for General Microbiology
Abstract Genotypic and phenotypic methods were applied to investigate differences between the closely related species Campylobacter hyoilei and Campylobacter coli. A unique DNA sequence from C. hyoilei was used to design a specific PCR assay that amplified a DNA product of 383 bp for all C. hyoilei strains, but not other Campylobacter species, including C. coli. The PCR assay could detect 100 fg pure C. hyoilei DNA, 2 x (10)2 c.f.u. ml(-1) using cultured cells and 8.3 x (10)3 c.f.u. 0.1 g(-1)in faeces. The C. hyoilei sequence utilized for specific detection and identification of this species showed similarities to sequences from bacteriophages Mu, P2 and 186, suggesting lysogination of the ancestral C. hyoilei genome. Activities of a set of 15 enzymes that participate in a variety of cellular functions, including biosynthesis, catabolism, energy generation, maintenance of redox balance and phosphate utilization, were tested using sets of strains of C. hyoilei and C. coli. Comparison of mean rates of enzyme activities revealed significant differences between species in the values determined for seven of these activities. Both the genetic and phenotypic data indicate that C. hyoilei is a unique Campylobacter species.
Copyright notice Copyright © 2001 by Society for General Microbiology
ISSN 1466-5026
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