The effect of ultrasound on casein micelle integrity

Chandrapala, J, Martin, G, Zisu, B, Kentish, S and Ashokkumar, M 2012, 'The effect of ultrasound on casein micelle integrity', Journal of Dairy Science, vol. 95, no. 12, pp. 6882-6890.


Document type: Journal Article
Collection: Journal Articles

Title The effect of ultrasound on casein micelle integrity
Author(s) Chandrapala, J
Martin, G
Zisu, B
Kentish, S
Ashokkumar, M
Year 2012
Journal name Journal of Dairy Science
Volume number 95
Issue number 12
Start page 6882
End page 6890
Total pages 9
Publisher Elsevier
Abstract Samples of fresh skim milk, reconstituted micellar casein, and casein powder were sonicated at 20 kHz to investigate the effect of ultrasonication. For fresh skim milk, the average size of the remaining fat globules was reduced by approximately 10 nm after 60 min of sonication; however, the size of the casein micelles was determined to be unchanged. A small increase in soluble whey protein and a corresponding decrease in viscosity also occurred within the first few minutes of sonication, which could be attributed to the breakup of casein-whey protein aggregates. No measurable changes in free casein content could be detected in ultracentrifuged skim milk samples sonicated for up to 60 min. A small, temporary decrease in pH resulted from sonication; however, no measurable change in soluble calcium concentration was observed. Therefore, casein micelles in fresh skim milk were stable during the exposure to ultrasonication. Similar results were obtained for reconstituted micellar casein, whereas larger viscosity changes were observed as whey protein content was increased. Controlled application of ultrasound can be usefully applied to reverse process-induced protein aggregation without affecting the native state of casein micelles.
Subject
Food Sciences
Keyword(s) ultrasonication
casein micelle
dynamic light scattering
viscosity
DOI - identifier 10.3168/jds.2012-5318
Copyright notice © American Dairy Science Association 2012
ISSN 0022-0302
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