Analytical approaches for the detailed characterization of microalgal lipid extracts for the production of biodiesel

Callahan, D, Martin, G, Hill, D, Olmstead, I and Dias, D 2015, 'Analytical approaches for the detailed characterization of microalgal lipid extracts for the production of biodiesel' in S.-K. Kim and K. Chojnacka (ed.) Marine Algae Extracts: Trend, Developments and Applications, Wiley-VCH Verlag GmbH and Co. KGaA, Germany, pp. 331-346.


Document type: Book Chapter
Collection: Book Chapters

Title Analytical approaches for the detailed characterization of microalgal lipid extracts for the production of biodiesel
Author(s) Callahan, D
Martin, G
Hill, D
Olmstead, I
Dias, D
Year 2015
Title of book Marine Algae Extracts: Trend, Developments and Applications
Publisher Wiley-VCH Verlag GmbH and Co. KGaA
Place of publication Germany
Editor(s) S.-K. Kim and K. Chojnacka
Start page 331
End page 346
Subjects Bioprocessing, Bioproduction and Bioproducts
Summary Many species of microalgae produce significant amounts of triacylglycerides (TAGs), the key product for biodiesel production. The lipid characteristics of microalgae are known to differ between species and change with growth conditions. Increasing TAG production by microalgae through optimization of growth regimes is of great interest as it is a convenient feedstock for biofuel production. Microalgae have the ability to synthesize and accumulate high levels of TAGs in specialized lipid bodies located in the cytoplasm and to a lesser extent in the chloroplast. In this chapter we first summarize the key lipids in microalgae, that is, the saponifiable lipids including neutral glycerolipids (NL; which include mono-, di-, and triglycerides) and some membrane-associated lipids including glycerophospholipids (PL), sphingolipids, and glycolipids (GL) as well as fatty acyls (free fatty acids). Aspects on gravimetric measurements, the simplest form of lipid characterization of total lipids, are presented followed by step-by-step protocols for the extraction and separation of lipid classes (NL, PL, and GL) using solid-phase extraction. The final discussion presents the preparation of lipid extracts for total and free fatty acid analysis using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC-MS) to determine the detailed composition of lipids within classes, also known as lipidomics. Screening microalgae for lipid species, searching those with high proportions of NL lipids, and determining strains and growth conditions that produce fatty acid profiles most suited for obtaining high quality biodiesel are also discussed. All these techniques are discussed in the content of biodiesel production.
Copyright notice © 2015 Wiley-VCH Verlag GmbH and Co. KGaA
DOI - identifier 10.1002/9783527679577.ch20
ISBN 9783527337088
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