Quantifying susceptibility of CD4+ stem memory T-cells to infection by laboratory adapted and clinical HIV-1 strains

Flynn, J, Paukovics, G, Cashin, K, Borm, K, Ellett, A, Roche, M, Jakobsen, M, Churchill, M and Gorry, P 2014, 'Quantifying susceptibility of CD4+ stem memory T-cells to infection by laboratory adapted and clinical HIV-1 strains', Viruses, vol. 6, no. 2, pp. 709-726.


Document type: Journal Article
Collection: Journal Articles

Title Quantifying susceptibility of CD4+ stem memory T-cells to infection by laboratory adapted and clinical HIV-1 strains
Author(s) Flynn, J
Paukovics, G
Cashin, K
Borm, K
Ellett, A
Roche, M
Jakobsen, M
Churchill, M
Gorry, P
Year 2014
Journal name Viruses
Volume number 6
Issue number 2
Start page 709
End page 726
Total pages 18
Publisher MDPI
Abstract CD4+ T cells are principal targets for human immunodeficiency virus type 1 (HIV-1) infection. CD4+ T cell subsets are heterogeneous cell populations, divided by functional and phenotypic differences into naïve and memory T cells. The memory CD4+ T cells are further segregated into central, effector and transitional memory cell subsets by functional, phenotypic and homeostatic characteristics. Defining the distribution of HIV-1 infection in different T cell subsets is important, as this can play a role in determining the size and composition of the viral reservoir. Both central memory and transitional memory CD4+ T cells have been described as long-lived viral reservoirs for HIV. Recently, the newly described stem memory T cell subset has also been implicated as a long-lived HIV reservoir. Using green fluorescent protein (GFP) reporter strains of HIV-1 and multi parameter flow cytometry, we developed an assay to simultaneously quantify the susceptibility of stem memory (TSCM), central memory, effector memory, transitional memory and naïve CD4+ T cell subsets, to HIV-1 infection in vitro. We show that TSCM are susceptible to infection with laboratory adapted and clinical HIV-1 strains. Our system facilitates the quantitation of HIV-1 infection in alternative T cell subsets by CCR5- and CXCR4-using viruses across different HIV-1 subtypes, and will be useful for studies of HIV-1 pathogenesis and viral reservoirs.
Subject Virology
Medical Virology
Infectious Diseases
Cellular Immunology
Keyword(s) HIV-1
stem memory T cells
CD4+ T cells
T cell subsets
envelope
viral reservoir
DOI - identifier 10.3390/v6020709
Copyright notice © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
ISSN 1999-4915
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