A simple and rapid colourimetric method for the determination of amylose in starch products

Mcgrane, S, Cornell, H and Rix, C 1998, 'A simple and rapid colourimetric method for the determination of amylose in starch products', Starch: International Journal for the Investigation, Processing and Use of Carbohydrates and Their Derivatives, vol. 50, no. 4, pp. 158-163.


Document type: Journal Article
Collection: Journal Articles

Title A simple and rapid colourimetric method for the determination of amylose in starch products
Author(s) Mcgrane, S
Cornell, H
Rix, C
Year 1998
Journal name Starch: International Journal for the Investigation, Processing and Use of Carbohydrates and Their Derivatives
Volume number 50
Issue number 4
Start page 158
End page 163
Total pages 5
Publisher Wiley - VCH Verlag GmbH and Co.
Abstract A method for the determination of the amylose content in starch has been developed which is based on the colorimetric measurement of the iodine complexes formed with amylose and amylopectin. The method requires measurement at only one wavelength and avoids the use of harsh dispersants for the starch. Dimethyl sulphoxide is used as the dispersant and a wavelength of 600 nm can be used for measurement of the amylose content of starches from different botanical sources. A linear relationship was obtained between absorbance and amylose concentration for mixtures of amylose and amylopectin standards, and this forms the basis of the determination. The method is rapid, simple, accurate and does not require the use of multi-component analysis of spectra, since a wavelength is chosen that suits the particular starch being analysed. It can be adapted to a micro-scale method if necessary.
Subject Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
DOI - identifier 10.1002/(SICI)1521-379X(199804)50:4
Copyright notice Copyright © 1998 Wiley - V C H Verlag GmbH & Co.
ISSN 0038-9056
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