Extraction of cereal prolamins and their toxicity in coeliac disease

McLachlan, A, Cullis, P and Cornell, H 2002, 'Extraction of cereal prolamins and their toxicity in coeliac disease', Journal of Biochemistry, Molecular Biology and Biophysics, vol. 6, no. 2, pp. 151-158.


Document type: Journal Article
Collection: Journal Articles

Title Extraction of cereal prolamins and their toxicity in coeliac disease
Author(s) McLachlan, A
Cullis, P
Cornell, H
Year 2002
Journal name Journal of Biochemistry, Molecular Biology and Biophysics
Volume number 6
Issue number 2
Start page 151
End page 158
Total pages 7
Publisher Taylor and Francis
Abstract Simple extraction of prolamins from cereal flour using 70% aqueous ethanol leads to co-extraction of lipids and other secondary products. Treatment of the crude extract with an excess of pure ethanol resulted in the removal of the majority of these compounds. Prolamin extracts obtained following ethanol precipitation showed little difference to products of more complex, multi stage, selective extractions, when compared using MALDI-TOF mass spectrometry. Toxicity tests on enzymic digests of ethanol-precipitated prolamins from coeliac-toxic cereals, coeliac-non-toxic cereals and oats were carried out using a rat liver lysosome assay. The prolamins from the ethanol-precipitated extracts showed greater activity than those extracted using only 70% ethanol for extraction. As the ethanol precipitation method is simple and provides a prolamin extract of sufficient purity for further evaluation, this procedure has been adopted as an alternative to more tedious procedures for preparation of cereal prolamins.
Subject Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Copyright notice © Cornell H.J.
ISSN 1025-8140
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 8 times in Scopus Article | Citations
Access Statistics: 250 Abstract Views  -  Detailed Statistics
Created: Mon, 11 Jan 2010, 09:45:29 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us