An integrated micromechanical large particle in flow sorter (MILPIS)

Mohd Fuad, N, Skommer, J, Friedrich, T, Kaslin, J and Wlodkowic, D 2015, 'An integrated micromechanical large particle in flow sorter (MILPIS)', in S. van den Driesche (ed.) Proceedings SPIE Vol. 9518 (Progress in Biomedical Optics and Imaging), Barcelona, Spain, 5-6 May 2015, pp. 951814-1-951814-10.


Document type: Conference Paper
Collection: Conference Papers

Title An integrated micromechanical large particle in flow sorter (MILPIS)
Author(s) Mohd Fuad, N
Skommer, J
Friedrich, T
Kaslin, J
Wlodkowic, D
Year 2015
Conference name Bio-MEMS and Medical Microdevices
Conference location Barcelona, Spain
Conference dates 5-6 May 2015
Proceedings title Proceedings SPIE Vol. 9518 (Progress in Biomedical Optics and Imaging)
Editor(s) S. van den Driesche
Publisher SPIE
Place of publication Bellingham, United States
Start page 951814-1
End page 951814-10
Total pages 10
Abstract At present, the major hurdle to widespread deployment of zebrafish embryo and larvae in large-scale drug development projects is lack of enabling high-throughput analytical platforms. In order to spearhead drug discovery with the use of zebrafish as a model, platforms need to integrate automated pre-test sorting of organisms (to ensure quality control and standardization) and their in-test positioning (suitable for high-content imaging) with modules for flexible drug delivery. The major obstacle hampering sorting of millimetre sized particles such as zebrafish embryos on chip-based devices is their substantial diameter (above one millimetre), mass (above one milligram), which both lead to rapid gravitational-induced sedimentation and high inertial forces. Manual procedures associated with sorting hundreds of embryos are very monotonous and as such prone to significant analytical errors due to operator's fatigue. In this work, we present an innovative design of a micromechanical large particle in-flow sorter (MILPIS) capable of analysing, sorting and dispensing living zebrafish embryos for drug discovery applications. The system consisted of a microfluidic network, revolving micromechanical receptacle actuated by robotic servomotor and opto-electronic sensing module. The prototypes were fabricated in poly(methyl methacrylate) (PMMA) transparent thermoplastic using infrared laser micromachining. Elements of MILPIS were also fabricated in an optically transparent VisiJet resin using 3D stereolithography (SLA) processes (ProJet 7000HD, 3D Systems). The device operation was based on a rapidly revolving miniaturized mechanical receptacle. The latter function was to hold and position individual fish embryos for (i) interrogation, (ii) sorting decision-making and (iii) physical sorting.. The system was designed to separate between fertilized (LIVE) and non-fertilized (DEAD) eggs, based on optical transparency using infrared (IR) emitters and receivers embedded
Subjects Microelectromechanical Systems (MEMS)
Animal Developmental and Reproductive Biology
Keyword(s) image acquisition
in-flow sorter
infrared sensors
zebrafish embryos
DOI - identifier 10.1117/12.2180691
Copyright notice © 2015 SPIE
ISSN 1605-7422
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