High resolution 3D imaging of synchrotron generated microbeams

Gagliardi, F, Cornelius, I, Blencowe, A, Franich, R and Geso, M 2015, 'High resolution 3D imaging of synchrotron generated microbeams', Medical Physics, vol. 42, no. 12, pp. 6973-6986.


Document type: Journal Article
Collection: Journal Articles

Title High resolution 3D imaging of synchrotron generated microbeams
Author(s) Gagliardi, F
Cornelius, I
Blencowe, A
Franich, R
Geso, M
Year 2015
Journal name Medical Physics
Volume number 42
Issue number 12
Start page 6973
End page 6986
Total pages 14
Publisher A I P Publishing LLC
Abstract Purpose: Microbeam radiation therapy (MRT) techniques are under investigation at synchrotrons worldwide. Favourable outcomes from animal and cell culture studies have proven the efficacy of MRT. The aim of MRT researchers currently is to progress to human clinical trials in the near future. The purpose of this study was to demonstrate the high resolution and 3D imaging of synchrotron generated microbeams in PRESAGE® dosimeters using laser fluorescence confocal microscopy. Methods: Water equivalent PRESAGE® dosimeters were fabricated and irradiated with microbeams on the Imaging and Medical Beamline at the Australian Synchrotron. Microbeam arrays comprised of microbeams 25-50 µm wide with 200 or 400 µm peak-to-peak spacing were delivered as single, cross-fire, multidirectional, and interspersed arrays. Imaging of the dosimeters was performed using a NIKON A 1 laser fluorescence confocal microscope. Results: The spatial fractionation of the MRT beams was clearly visible in 2D and up to 9 mm in depth. Individual microbeams were easily resolved with the full width at half maximum of microbeams measured on images with resolutions of as low as 0.09 µm/pixel. Profiles obtained demonstrated the change of the peak-to-valley dose ratio for interspersed MRT microbeam arrays and subtle variations in the sample positioning by the sample stage goniometer were measured. Conclusions: Laser fluorescence confocal microscopy of MRT irradiated PRESAGE® dosimeters has been validated in this study as a high resolution imaging tool for the independent spatial and geometrical verification of MRT beam delivery.
Subject Physical Sciences not elsewhere classified
Keyword(s) DynMRT
IntMRT
microbeam imaging
MRT
PRESAGE® dosimeter
DOI - identifier 10.1118/1.4935410
Copyright notice © 2015 American Association of Physicists in Medicine
ISSN 0094-2405
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