A multimode-TIRFM and microfluidic technique to examine platelet adhesion dynamics

Nesbitt, W, Tovar Lopez, F, Westein, E, Harper, I and Jackson, S 2013, 'A multimode-TIRFM and microfluidic technique to examine platelet adhesion dynamics' in Amanda S. Coutts (ed.) Adhesion Protein Protocols, Humana Press, United Kingdom, pp. 39-58.

Document type: Book Chapter
Collection: Book Chapters

Title A multimode-TIRFM and microfluidic technique to examine platelet adhesion dynamics
Author(s) Nesbitt, W
Tovar Lopez, F
Westein, E
Harper, I
Jackson, S
Year 2013
Title of book Adhesion Protein Protocols
Publisher Humana Press
Place of publication United Kingdom
Editor(s) Amanda S. Coutts
Start page 39
End page 58
Subjects Biomechanical Engineering
Signal Transduction
Numerical Modelling and Mechanical Characterisation
Summary Fluorescence microscopy techniques have provided important insights into the structural and signalling events occurring during platelet adhesion under both static and blood flow conditions. However, due to limitations in sectioning ability and sensitivity these techniques are restricted in their capacity to precisely image the adhesion footprint of spreading platelets. In particular, investigation of platelet adhesion under hemodynamic shear stress requires an imaging platform with high spatial discrimination and sensitivity and rapid temporal resolution. This chapter describes in detail a multimode imaging approach combining total internal reflection fluorescence microscopy (TIRFM) with high speed epifluorescence and differential interference contrast (DIC) microscopy along with a novel microfluidic perfusion system developed in our laboratory to examine platelet membrane adhesion dynamics under static and flow conditions.
Copyright notice © Springer Science+Business Media, LLC 2013
Keyword(s) Platelets TIRFM Microscopy Adhesion Hemodynamics
DOI - identifier 10.1007/978-1-62703-538-5_3
ISBN 9781627035378
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