Central administration of insulin and leptin together enhance renal sympathetic nerve activity and Fos production in the arcuate nucleus

Habeeballah, H, Alsuhaymi, N, Stebbing, M, Jenkins, T and Badoer, E 2017, 'Central administration of insulin and leptin together enhance renal sympathetic nerve activity and Fos production in the arcuate nucleus', Frontiers in Physiology, vol. 7, no. 1, 672, pp. 1-9.


Document type: Journal Article
Collection: Journal Articles

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Title Central administration of insulin and leptin together enhance renal sympathetic nerve activity and Fos production in the arcuate nucleus
Author(s) Habeeballah, H
Alsuhaymi, N
Stebbing, M
Jenkins, T
Badoer, E
Year 2017
Journal name Frontiers in Physiology
Volume number 7
Issue number 1
Article Number 672
Start page 1
End page 9
Total pages 9
Publisher Frontiers Research Foundation
Abstract There is considerable interest in the central actions of insulin and leptin. Both induce sympatho-excitation. This study (i) investigated whether centrally administered leptin and insulin together elicits greater increases in renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) than when given alone, and (ii) quantified the number of activated neurons in brain regions influencing SNA, to identify potential central sites of interaction. In anesthetised (urethane 1.4-1.6 g/kg iv) male Sprague-Dawley rats, RSNA, MAP, and HR were recorded following intracerebroventricular (ICV) saline (control; n = 5), leptin (7 μg; n = 5), insulin (500 mU; n = 4) and the combination of leptin and insulin; (n = 4). Following leptin or insulin alone, RSNA was significantly increased (74 and 62% respectively). MAP responses were not significantly different between the groups. Insulin alone significantly increased HR. Leptin alone also increased HR but it was significantly less than following insulin alone (P < 0.005). When leptin and insulin were combined, the RSNA increase (124%) was significantly greater than the response to either alone. There were no differences between the groups in MAP responses, however, the increase in HR induced by insulin was attenuated by leptin. Of the brain regions examined, only in the arcuate nucleus did leptin and insulin together increase the number of Fos-positive cell nuclei significantly more than leptin or insulin alone. In the lamina terminalis and rostroventrolateral medulla, leptin and insulin together increased Fos, but the effect was not greater than leptin alone. The results suggest that when central leptin and insulin levels are elevated, the sympatho-excitatory response in RSNA will be greater. The arcuate nucleus may be a common site of cardiovascular integration.
Subject Cardiology (incl. Cardiovascular Diseases)
Basic Pharmacology
Keyword(s) Cardiovascular function
Central interactions
Insulin
Leptin
Renal sympathetic nerve activity
DOI - identifier 10.3389/fphys.2016.00672
Copyright notice © 2017 Habeeballah, Alsuhaymi, Stebbing, Jenkins and Badoer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
ISSN 1664-042X
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