Histochemical localization of apoptosis with in situ labeling of fragmented DNA

Hewitson, T, Bisucci, T and Darby, I 2006, 'Histochemical localization of apoptosis with in situ labeling of fragmented DNA' in I. Darby, T. Hewitson (ed.) Methods in Molecular Biology/In Situ Hybridization Protocols, Humana Press, Totowa, NJ, pp. 227-234.


Document type: Book Chapter
Collection: Book Chapters

Title Histochemical localization of apoptosis with in situ labeling of fragmented DNA
Author(s) Hewitson, T
Bisucci, T
Darby, I
Year 2006
Title of book Methods in Molecular Biology/In Situ Hybridization Protocols
Publisher Humana Press
Place of publication Totowa, NJ
Editor(s) I. Darby, T. Hewitson
Start page 227
End page 234
Subjects Cell Physiology
Summary Cell death by apoptosis is now recognized widely as an important constituent of cell turnover and disease pathology. Characterized by the cleavage of DNA into oligonucleosome-sized DNA fragments, end-labeling of fragmented DNA often is used as an in situ histological marker of apoptosis. The judicious and appropriate use of this technique therefore provides us with an important tool for assessing cell kinetics. Protocols for both terminal transferase-mediated UTP nick end-labeling, so-called TUNEL, and the combination of TUNEL with immunohistochemical staining are presented here, along with a discussion of its significance and interpretation.
Copyright notice © 2006 Humana Press Inc.
Keyword(s) Apoptosis; fragmented DNA; cell death; tunel
DOI - identifier 10.1385/1-59745-007-3:227
ISBN 9780896036864
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