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Histochemical localization of cell proliferation using in situ hybridization for histone mRNA Hewitson, T, Kelynack, K and Darby, I 2006, 'Histochemical localization of cell proliferation using in situ hybridization for histone mRNA' in I. Darby and T Hewitson (ed.) Methods in Molecular Biology/In Situ Hybridization Protocols, Humana Press, Totowa, NJ, pp. 219-226. Document type: Book Chapter Collection: Book Chapters Title Histochemical localization of cell proliferation using in situ hybridization for histone mRNA Author(s) Hewitson, T Kelynack, K Darby, I Year 2006 Title of book Methods in Molecular Biology/In Situ Hybridization Protocols Publisher Humana Press Place of publication Totowa, NJ Editor(s) I. Darby and T Hewitson Start page 219 End page 226 Subjects Cell Development, Proliferation and Death Summary Monoclonal antibodies to proliferation associated antigens have long been used to histologically localize mitogenesis. However, techniques that distinguish cells in the synthetic or S phase have tended to rely on the in vivo incorporation of tritiated thymidine or thymidine analogs such as bromodeoxyuridine. The necessity to pulse with these labels before retrieving tissue means that they cannot be used in humans and are not available retrospectively. Measuring expression of histones serves as a useful adjunct to these techniques. As expression of histone proteins (H2A, H2B, H3, H4) are restricted to the synthetic phase of the cell cycle, hybridization for histone mRNA precisely distinguishes those cells in the S phase. Measuring their expression can easily be applied to the histological localization of proliferation, and can be used both prospectively and with archived tissue specimens. Several histone in situ hybridization probes and nonradioactive detection systems are now available commercially. A generalized protocol for their use in measuring in situ proliferation is provided in this chapter. Copyright notice © Humana Press Inc Keyword(s) Proliferation mitogenesis histone cell cycle in situ hybridization ISBN 9781588294029 Related Links Link Description http://trove.nla.gov.au/work/9420152 Find out more >>   Versions Version Filter Type Mon, 20 Sep 2010, 12:10:19 EST Filtered Full Access Statistics: 221 Abstract Views  -  Detailed Statistics Created: Mon, 20 Sep 2010, 10:56:00 EST by Catalyst Administrator