Histochemical localization of cell proliferation using in situ hybridization for histone mRNA

Hewitson, T, Kelynack, K and Darby, I 2006, 'Histochemical localization of cell proliferation using in situ hybridization for histone mRNA' in I. Darby and T Hewitson (ed.) Methods in Molecular Biology/In Situ Hybridization Protocols, Humana Press, Totowa, NJ, pp. 219-226.


Document type: Book Chapter
Collection: Book Chapters

Title Histochemical localization of cell proliferation using in situ hybridization for histone mRNA
Author(s) Hewitson, T
Kelynack, K
Darby, I
Year 2006
Title of book Methods in Molecular Biology/In Situ Hybridization Protocols
Publisher Humana Press
Place of publication Totowa, NJ
Editor(s) I. Darby and T Hewitson
Start page 219
End page 226
Subjects Cell Development, Proliferation and Death
Summary Monoclonal antibodies to proliferation associated antigens have long been used to histologically localize mitogenesis. However, techniques that distinguish cells in the synthetic or S phase have tended to rely on the in vivo incorporation of tritiated thymidine or thymidine analogs such as bromodeoxyuridine. The necessity to pulse with these labels before retrieving tissue means that they cannot be used in humans and are not available retrospectively. Measuring expression of histones serves as a useful adjunct to these techniques. As expression of histone proteins (H2A, H2B, H3, H4) are restricted to the synthetic phase of the cell cycle, hybridization for histone mRNA precisely distinguishes those cells in the S phase. Measuring their expression can easily be applied to the histological localization of proliferation, and can be used both prospectively and with archived tissue specimens. Several histone in situ hybridization probes and nonradioactive detection systems are now available commercially. A generalized protocol for their use in measuring in situ proliferation is provided in this chapter.
Copyright notice © Humana Press Inc
Keyword(s) Proliferation
mitogenesis
histone
cell cycle
in situ hybridization
ISBN 9781588294029
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