Shear stress regulates TRPV4 channel clustering and translocation from adherens junctions to the basal membrane

Baratchi, S, Knoerzer, M, Khoshmanesh, K, Mitchell, A and McIntyre, P 2017, 'Shear stress regulates TRPV4 channel clustering and translocation from adherens junctions to the basal membrane', Scientific Reports, vol. 7, 15942, pp. 1-9.


Document type: Journal Article
Collection: Journal Articles

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Title Shear stress regulates TRPV4 channel clustering and translocation from adherens junctions to the basal membrane
Author(s) Baratchi, S
Knoerzer, M
Khoshmanesh, K
Mitchell, A
McIntyre, P
Year 2017
Journal name Scientific Reports
Volume number 7
Article Number 15942
Start page 1
End page 9
Total pages 9
Publisher Nature Publishing Group
Abstract Localized Ca2+ influx via TRPV4 on the surface of endothelial cells greatly influences endothelial adaptation to blood flow, but how mechanical stress from blood flow controls TRPV4 integration into this physiological function is not fully understood. Here, we studied the spatial organization of TRPV4 and its relationship to the adherens junction component beta-catenin using single-and dual-color direct stochastic optical reconstruction microscopy (dSTORM). In non-stimulated endothelial cells, TRPV4 is clustered in small protein islands, as is beta-catenin. Using dual-color imaging, we found that TRPV4 and beta-catenin reside in similar islands and can be found at both the basolateral and basal membranes. Following shear stress stimulation, TRPV4 molecules formed smaller clusters, with the majority residing outside of clusters. Further shear stress stimulation changed the molecular distribution of TRPV4 molecules, limiting them to the basal membrane. This redistribution and the smaller clusters resulted in the segregation of TRPV4 from beta-catenin. Furthermore, TRPV4 trafficking was controlled by focal adhesion kinase and activation of the alpha 5 beta 1 integrin. These highly differentiated spatial redistributions suggest that mechanotransduction of blood flow is controlled via a more complex hierarchy than previously thought.
Subject Receptors and Membrane Biology
Keyword(s) Vascular Endothelial-Cells
Adhesion Receptors
Integrins
Mechanotransduction
Activation
Kinase
Influx
DOI - identifier 10.1038/s41598-017-16276-7
Copyright notice © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.To view a copy of this license, visit: http://creativecommons.org/licenses/by/4.0/.
ISSN 2045-2322
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