Competitive inhibition of the enzyme-mimic activity of Gd-based nanorods toward highly specific colorimetric sensing of L cysteine

Singh, M, WEERATHUNGE, P, Balapitiya Liyanage, P, Mayes, E, Ramanathan, R and Bansal, V 2017, 'Competitive inhibition of the enzyme-mimic activity of Gd-based nanorods toward highly specific colorimetric sensing of L cysteine', Langmuir, vol. 33, no. 38, pp. 10006-10015.


Document type: Journal Article
Collection: Journal Articles

Title Competitive inhibition of the enzyme-mimic activity of Gd-based nanorods toward highly specific colorimetric sensing of L cysteine
Author(s) Singh, M
WEERATHUNGE, P
Balapitiya Liyanage, P
Mayes, E
Ramanathan, R
Bansal, V
Year 2017
Journal name Langmuir
Volume number 33
Issue number 38
Start page 10006
End page 10015
Total pages 10
Publisher American Chemical Society
Abstract Gd-based nanomaterials offer interesting magnetic properties and have been heavily investigated for magnetic resonance imaging. The applicability of these materials beyond biomedical imaging remains limited. The current study explores the applicability of these rare-earth nanomaterials as nanozyme-mediated catalysts for colorimetric sensing of l-cysteine, an amino acid of high biomedical relevance. We show a facile solution-based strategy to synthesize two Gd-based nanomaterials viz. Gd(OH) 3 and Gd 2 O 3 nanorods. We further establish the catalytic peroxidase-mimic nanozyme activity of these Gd(OH) 3 and Gd 2 O 3 nanorods. This catalytic activity was suppressed specifically in the presence of l-cysteine that allowed us to develop a colorimetric sensor to detect this biologically relevant molecule among various other contaminants. This suppression, which could either be caused due to catalyst poisoning or enzyme inhibition, prompted extensive investigation of the kinetics of this catalytic inhibition in the presence of cysteine. This revealed a competitive inhibition process, a mechanism akin to those observed in natural enzymes, bringing nanozymes a step closer to the biological systems.
Subject Philosophy and Religious Studies not elsewhere classified
Keyword(s) Peroxidase-Like Activity
Performance Liquid-Chromatography
Mri Contrast Agents
Co3o4 Nanoparticles
Amino-Acids
Catalytic-Activity
Hydrogen-Peroxide
Oxide
Glucose
Homocysteine
DOI - identifier 10.1021/acs.langmuir.7b01926
Copyright notice © 2017 American Chemical Society.
ISSN 0743-7463
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