Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation

Wang, H, Wilksch, J, Chen, L, Tan, J, Strugnell, R and Gee, M 2016, 'Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation', Langmuir, vol. 33, no. 1, pp. 100-106.


Document type: Journal Article
Collection: Journal Articles

Title Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation
Author(s) Wang, H
Wilksch, J
Chen, L
Tan, J
Strugnell, R
Gee, M
Year 2016
Journal name Langmuir
Volume number 33
Issue number 1
Start page 100
End page 106
Total pages 7
Publisher American Chemical Society
Abstract The surface polymers of bacteria determine the ability of bacteria to adhere to a substrate for colonization, which is an essential step for a variety of microbial processes, such as biofilm formation and biofouling. Capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell-surface interactions. Adhesion and viscoelasticity of bacteria are two major physical properties related to bacteria-surface interactions. In this study, we employed atomic force microscopy (AFM) to interrogate how the adhesion work and the viscoelasticity of a bacterial pathogen, Klebsiella pneumoniae, influence biofilm formation. To do this, the wild-type, type 3 fimbriae-deficient, and type 3 fimbriae-overexpressed K. pneumoniae strains have been investigated in an aqueous environment. The results show that the measured adhesion work is positively correlated to biofilm formation; however, the viscoelasticity is not correlated to biofilm formation. This study indicates that AFM-based adhesion measurements of bacteria can be used to evaluate the function of bacterial surface polymers in biofilm formation and to predict the ability of bacterial biofilm formation.
Subject Biomedical Engineering not elsewhere classified
DOI - identifier 10.1021/acs.langmuir.6b03764
Copyright notice © 2016 American Chemical Society
ISSN 0743-7463
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