Glucose transporter 1-expressing proinflammatory monocytes are elevated in combination antiretroviral therapy-treated and untreated HIV+ subjects

Palmer, C, Anzinger, J, Zhou, J, Gouillou, M, Landay, A, Jaworowski, A, McCune, J and Crowe, S 2014, 'Glucose transporter 1-expressing proinflammatory monocytes are elevated in combination antiretroviral therapy-treated and untreated HIV+ subjects', Journal of Immunology, vol. 193, no. 11, pp. 5595-5603.


Document type: Journal Article
Collection: Journal Articles

Title Glucose transporter 1-expressing proinflammatory monocytes are elevated in combination antiretroviral therapy-treated and untreated HIV+ subjects
Author(s) Palmer, C
Anzinger, J
Zhou, J
Gouillou, M
Landay, A
Jaworowski, A
McCune, J
Crowe, S
Year 2014
Journal name Journal of Immunology
Volume number 193
Issue number 11
Start page 5595
End page 5603
Total pages 9
Publisher American Association of Immunologists
Abstract Monocyte activation during HIV-1 infection is associated with increased plasma levels of inflammatory markers and increased risk for premature development of age-related diseases. Because activated monocytes primarily use glucose to support cellular metabolism, we hypothesized that chronicmonocyte activation duringHIV-1 infection induces a hypermetabolic response with increased glucose uptake. To test this hypothesis, we evaluated glucose transporter 1 (Glut1) expression and glucose uptake by monocyte subpopulations in HIVseropositive (HIV < sup > + < /sup > ) treatment-naive individuals (n = 17), HIV < sup > + < /sup > individuals on combination antiretroviral therapy with viral loads below detection (n = 11), and HIV-seronegative (HIV < sup > - < /sup > ) individuals (n = 16). Surface expression of Glut1 and cellular uptake of the fluorescent glucose analog 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2 deoxyglucose were analyzed by flow cytometry on monocyte subpopulations. Irrespective of treatment status, monocytes from HIV < sup > + < /sup > persons had significantly increased surface expression ofGlut1 compared with those from HIV < sup > - < /sup > controls. Nonclassical (CD14 < sup > + < /sup > CD16 < sup > ++ < /sup > ) and intermediate (CD14 < sup > ++ < /sup > CD16 < sup > + < /sup > ) monocyte subpopulations showed higher Glut1 expression than did classical (CD14 < sup > ++ < /sup > CD16 < sup > - < /sup > ) monocytes. Intermediate monocytes from treatment-naive HIV < sup > + < /sup > individuals also showed increased uptake of 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2 deoxyglucose compared with those from HIV < sup > - < /sup > controls. Our results show that HIV infection is associated with increased glucose metabolism in monocytes and that Glut1 expression by proinflammatory monocytes is a pote
Subject Infectious Diseases
Innate Immunity
DOI - identifier 10.4049/jimmunol.1303092
Copyright notice © 2014 by The American Association of Immunologists, Inc.
ISSN 0022-1767
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