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Proteome-wide mapping of immune features onto Plasmodium protein three-dimensional structures Guy, A, Irani, V, Beeson, J, Webb, B, Sali, A, Richards, J and Ramsland, P 2018, 'Proteome-wide mapping of immune features onto Plasmodium protein three-dimensional structures', Scientific Reports, vol. 8, 4355, pp. 1-16. Document type: Journal Article Collection: Journal Articles Title Proteome-wide mapping of immune features onto Plasmodium protein three-dimensional structures Author(s) Guy, A Irani, V Beeson, J Webb, B Sali, A Richards, J Ramsland, P Year 2018 Journal name Scientific Reports Volume number 8 Article Number 4355 Start page 1 End page 16 Total pages 16 Publisher Nature Publishing Group Abstract Humoral immune responses against the malaria parasite are an important component of a protective immune response. Antibodies are often directed towards conformational epitopes, and the native structure of the antigenic region is usually critical for antibody recognition. We examined the structural features of various Plasmodium antigens that may impact on epitope location, by performing a comprehensive analysis of known and modelled structures from P. falciparum. Examining the location of known polymorphisms over all available structures, we observed a strong propensity for polymorphic residues to be exposed on the surface and to occur in particular secondary structure segments such as hydrogen-bonded turns. We also utilised established prediction algorithms for B-cell epitopes and MHC class II binding peptides, examining predicted epitopes in relation to known polymorphic sites within structured regions. Finally, we used the available structures to examine polymorphic hotspots and Tajima's D values using a spatial averaging approach. We identified a region of PfAMA1 involving both domains II and III under a high degree of balancing selection relative to the rest of the protein. In summary, we developed general methods for examining how sequence-based features relate to one another in three-dimensional space and applied these methods to key P. falciparum antigens. Subject Structural Biology (incl. Macromolecular Modelling) Infectious Agents DOI - identifier 10.1038/s41598-018-22592-3 Copyright notice This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018 ISSN 2045-2322 Related Links Link Description https://dx.doi.org/10.1038/s41598-018-22592-3 Link to Published Version   Versions Version Filter Type Thu, 20 Sep 2018, 13:37:14 EST Wed, 03 Oct 2018, 14:44:47 EST Filtered Full Citation counts:   Cited 5 times in Thomson Reuters Web of Science Article | Citations Cited 0 times in Scopus Article Altmetric details: Access Statistics: 31 Abstract Views  -  Detailed Statistics Created: Wed, 19 Sep 2018, 13:27:00 EST by Catalyst Administrator