Talaromyces marneffei simA encodes a fungal cytochrome P450 essential for survival in macrophages

Boyce, K, De Souza, D, Dayalan, S, Pasricha, S, Tull, D, McConville, M and Andrianopoulos, A 2018, 'Talaromyces marneffei simA encodes a fungal cytochrome P450 essential for survival in macrophages', mSphere, vol. 3, no. 2, pp. 1-18.


Document type: Journal Article
Collection: Journal Articles

Title Talaromyces marneffei simA encodes a fungal cytochrome P450 essential for survival in macrophages
Author(s) Boyce, K
De Souza, D
Dayalan, S
Pasricha, S
Tull, D
McConville, M
Andrianopoulos, A
Year 2018
Journal name mSphere
Volume number 3
Issue number 2
Start page 1
End page 18
Total pages 18
Publisher American Society for Microbiology
Abstract Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes the characterization of a yeast-specific CYP encoded by simA ("survival in macrophages"). Deletion of simA does not affect yeast growth at 37°C in vitro but is essential for yeast cell production during macrophage infection. The ?simA strain exhibits reduced conidial germination and intracellular growth of yeast in macrophages, suggesting that the enzymatic product of SimA is required for normal fungal growth in vivo. Intracellular ?simA yeast cells exhibit cell wall defects, and metabolomic and chemical sensitivity data suggest that SimA may promote chitin synthesis or deposition in vitro. In vivo, ?simA yeast cells subsequently lyse and are degraded, suggesting that SimA may increase resistance to and/or suppress host cell biocidal effectors. The results suggest that simA synthesizes a secondary metabolite that allows T. marneffei to occupy the specific intracellular environmental niche within the macrophage. © 2018 Boyce et al.
Subject Mycology
Infectious Diseases
Medical Microbiology not elsewhere classified
Keyword(s) Dimorphism
Host-pathogen interactions
Mycology
DOI - identifier 10.1128/mSphere.00056-18
Copyright notice Copyright © 2018 Boyce et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
ISSN 2379-5042
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