The Akt signaling pathway in skin cancer cell lines treated with RRM-MV, a short peptide analogue of the MT5 protein of myxoma virus

Istivan, T, ALMANSOUR, N and Pirogova, E 2018, 'The Akt signaling pathway in skin cancer cell lines treated with RRM-MV, a short peptide analogue of the MT5 protein of myxoma virus', Cancer Studies and Therapeutics, vol. 3, no. 2, pp. 1-10.


Document type: Journal Article
Collection: Journal Articles

Title The Akt signaling pathway in skin cancer cell lines treated with RRM-MV, a short peptide analogue of the MT5 protein of myxoma virus
Author(s) Istivan, T
ALMANSOUR, N
Pirogova, E
Year 2018
Journal name Cancer Studies and Therapeutics
Volume number 3
Issue number 2
Start page 1
End page 10
Total pages 10
Publisher Research Open World
Abstract In silico methods can help in identifying drug targets via bioinformatics tools. In our previous studies, a 2.34 kDa short linear peptide analogue to myxoma virus M-T5 protein was computationally designed using the resonant recognition model (RRM). Quantitative and qualitative cell cytotoxicity assays showed that the peptide analogue can induce a dose and time dependent tumoricidal effect on skin cancer cells but not on normal cells. It has been reported that the viral protein (M-T5) binds with p-Akt to regulate Akt signaling in some human cancer types. Hence in this study we investigated the effects induced by the peptide analogue RRM- MV on the Akt pathway in skin cancer and normal cells. Akt expression levels in cells treated with RRM-MV, in the presence or absence of PI3K/Akt inhibitor, were detected in mammalian skin cancer and normal cells using immunoblotting. The results revealed that different endogenous levels of p-Akt were expressed in the human melanoma and carcinoma cells following treatment with RRM-MV. Yet, it did not appear to affect the p-Akt expression in the mouse melanoma cells. Furthermore, RRM-MV treatment did not seem to affect the level of total Akt in any type of cancer cells under the same experimental conditions. Hence, the bioactive peptide RRM-MV appears to be targeting the Akt pathway in some types of skin cancer cells. Elucidating the mechanism of RRM-MV effects on other cellular apoptosis pathways requires further investigation.
Subject Cell Development, Proliferation and Death
Biomechanical Engineering
Cancer Cell Biology
Keyword(s) Akt
RRM-MV
Cell death pathway
Carcinoma
Melanoma
Myxoma virus.
ISSN 2002-7184
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