'Candidatus Phytoplasma wodyetiae', a new taxon associated with yellow decline disease of foxtail palm (Wodyetia bifurcata) in Malaysia

Naderali, N, Nejat, N, Vadamalai, G, Davis, R, Wei, W, Harrison, N, Kong, L, Kadir, J, Tan, Y and Zhao, Y 2017, ''Candidatus Phytoplasma wodyetiae', a new taxon associated with yellow decline disease of foxtail palm (Wodyetia bifurcata) in Malaysia', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 10, pp. 3765-3772.


Document type: Journal Article
Collection: Journal Articles

Title 'Candidatus Phytoplasma wodyetiae', a new taxon associated with yellow decline disease of foxtail palm (Wodyetia bifurcata) in Malaysia
Author(s) Naderali, N
Nejat, N
Vadamalai, G
Davis, R
Wei, W
Harrison, N
Kong, L
Kadir, J
Tan, Y
Zhao, Y
Year 2017
Journal name International Journal of Systematic and Evolutionary Microbiology
Volume number 67
Issue number 10
Start page 3765
End page 3772
Total pages 8
Publisher Microbiology Society
Abstract Landscape-grown foxtail palm (Wodyetia bifurcata A. K. Irvine) trees displaying symptoms of severe foliar chlorosis, stunting, general decline and mortality reminiscent of coconut yellow decline disease were observed in Bangi, Malaysia, during 2012. DNA samples from foliage tissues of 15 symptomatic palms were analysed by employing a nested PCR assay primed by phytoplasma universal ribosomal RNA operon primer pairs, P1/P7 followed by R16F2n/R2. The assay yielded amplicons of a single band of 1.25kb from DNA samples of 11 symptomatic palms. Results from cloning and sequence analysis of the PCR-amplified 16S rRNA gene segments revealed that, in three palms, three mutually distinct phytoplasmas comprising strains related to Candidatus Phytoplasma asteris and Candidatus Phytoplasma cynodontis, as well as a novel phytoplasma, were present as triple infections. The 16S rRNA gene sequence derived from the novel phytoplasma shared less than 96 % nucleotide sequence identity with that of each previously describedspecies of the provisional genus Ca. Phytoplasma , justifying its recognition as the reference strain of a new taxon, Candidatus Phytoplasma wodyetiae. Virtual RFLP profiles of the R16F2n/R2 portion of the 16S rRNA gene and the pattern similarity coefficient value (0.74) supported the delineation of Ca. Phytoplasma wodyetiae as the sole representative subgroup A member of a new phytoplasma ribosomal group, 16SrXXXVI.
Subject Microbiology not elsewhere classified
Evolutionary Biology not elsewhere classified
Keyword(s) Foxtail palm
Mixed infection
Phytoplasma
Yellow decline disease
DOI - identifier 10.1099/ijsem.0.002187
Copyright notice © 2017 IUMS.
ISSN 1466-5026
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