Fluorescent single-digit detonation nanodiamond for biomedical applications

Nunn, N, d'Amora, M, Prabhakar, N, Panich, A, Froumin, N, Torelli, M, Vlasov, I, Reineck, P, Gibson, B, Rosenholm, J, Giordani, S and Shenderova, O 2018, 'Fluorescent single-digit detonation nanodiamond for biomedical applications', Methods and Applications in Fluorescence, vol. 6, no. 3, pp. 1-10.

Document type: Journal Article
Collection: Journal Articles

Title Fluorescent single-digit detonation nanodiamond for biomedical applications
Author(s) Nunn, N
d'Amora, M
Prabhakar, N
Panich, A
Froumin, N
Torelli, M
Vlasov, I
Reineck, P
Gibson, B
Rosenholm, J
Giordani, S
Shenderova, O
Year 2018
Journal name Methods and Applications in Fluorescence
Volume number 6
Issue number 3
Start page 1
End page 10
Total pages 10
Publisher Institute of Physics Publishing Ltd.
Abstract Detonation nanodiamonds (DNDs) have emerged as promising candidates for a variety of biomedical applications, thanks to different physicochemical and biological properties, such as small size and reactive surfaces. In this study, we propose carbon dot decorated single digit (4-5 nmdiameter) primary particles of detonation nanodiamond as promising fluorescent probes. Due to their intrinsic fluorescence originating from tiny (1-2 atomic layer thickness) carbonaceous structures on their surfaces, they exhibit brightness suitable for in vitro imaging. Moreover, this material offers a unique, cost effective alternative to sub-10 nmnanodiamonds containing fluorescent nitrogen-vacancy color centers, which have not yet been produced at large scale. In this paper, carbon dot decorated nanodiamonds are characterized by several analytical techniques. In addition, the efficient cellular uptake and fluorescence of these particles are observed in vitro on MDA-MD-231 breast cancer cells by means of confocal imaging. Finally, the in vivo biocompatibility of carbon dot decorated nanodiamonds is demonstrated in zebrafish during the development. Our results indicate the potential of single-digit detonation nanodiamonds as biocompatible fluorescent probes. This unique material will find application in correlative light and electron microscopy, where small sized NDs can be attached to antibodies to act as a suitable dual marker for intracellular correlative microscopy of biomolecules.
Subject Condensed Matter Imaging
DOI - identifier 10.1088/2050-6120/aac0c8
Copyright notice © 2018 IOP Publishing Ltd
ISSN 2050-6120
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