Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study

Wang, H, Wilksch, J, Strugnell, R and Gee, M 2015, 'Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study', ACS Applied Materials and Interfaces, vol. 7, no. 23, pp. 13007-13013.


Document type: Journal Article
Collection: Journal Articles

Title Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study
Author(s) Wang, H
Wilksch, J
Strugnell, R
Gee, M
Year 2015
Journal name ACS Applied Materials and Interfaces
Volume number 7
Issue number 23
Start page 13007
End page 13013
Total pages 7
Publisher American Chemical Society
Abstract Bacteria form biofilms to facilitate colonization of biotic and abiotic surfaces, and biofilm formation on indwelling medical devices is a common cause of hospital-acquired infection. Although it is well-recognized that the exopolysaccharide capsule is one of the key bacterial components for biofilm formation, the underlying biophysical mechanism is poorly understood. In the present study, nanomechanical measurements of wild type and specific mutants of the pathogen, Klebsiella pneumoniae, were performed in situ using atomic force microscopy (AFM). Theoretical modeling of the mechanical data and static microtiter plate biofilm assays show that the organization of the capsule can influence bacterial adhesion, and thereby biofilm formation. The capsular organization is affected by the presence of type 3 fimbriae. Understanding the biophysical mechanisms for the impact of the structural organization of the bacterial polysaccharide capsule on biofilm formation will aid the development of strategies to prevent biofilm formation.
Subject Mechanical Engineering not elsewhere classified
Keyword(s) bacteria
biofilm
capsule
fimbriae
nanomechanics
polysaccharides
DOI - identifier 10.1021/acsami.5b03041
Copyright notice © 2015 American Chemical Society
ISSN 1944-8244
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