Isolation and clonal assay of adult lung epithelial stem/progenitor cells

Bertoncello, I and McQualter, J 2011, 'Isolation and clonal assay of adult lung epithelial stem/progenitor cells', American Journal of Medical Genetics, pp. 1-12.

Document type: Journal Article
Collection: Journal Articles

Title Isolation and clonal assay of adult lung epithelial stem/progenitor cells
Author(s) Bertoncello, I
McQualter, J
Year 2011
Journal name American Journal of Medical Genetics
Start page 1
End page 12
Total pages 12
Publisher John Wiley and Sons
Abstract Adult mouse lung epithelial stem/progenitor cells (EpiSPC) can be defined in vitro as epithelial colony-forming units that are capable of self-renewal, and which when co-cultured with lung mesenchymal stromal cells (MSC) are able to give rise to differentiated progeny comprising mature lung epithelial cells. This unit describes a protocol for the prospective isolation and in vitro propagation and differentiation of adult mouse lung EpiSPC. The strategy used for selection of EpiSPC and MSC from adult mouse lung by enzymatic digestion and flow cytometry is based on the differential expression of CD45, CD31, Sca-1, EpCAM, and CD24. The culture conditions required for the differentiation (co-culture with MSC) and expansion (stromal-free culture with FGF-10 and HGF) of EpiSPC are described.
Subject Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
Keyword(s) lung epithelium
stem cells
colony-forming assay
DOI - identifier 10.1002/9780470151808.sc02g01s16
Copyright notice © 2011 by John Wiley and Sons, Inc.
ISSN 1552-4841
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