Direct visualization of the structural transformation between the lyotropic liquid crystalline lamellar and bicontinuous cubic mesophase

Tran, N, Zhai, J, Conn, C, Mulet, X, Waddington, L and Drummond, C 2018, 'Direct visualization of the structural transformation between the lyotropic liquid crystalline lamellar and bicontinuous cubic mesophase', Journal of Physical Chemistry Letters, vol. 9, no. 12, pp. 3397-3402.


Document type: Journal Article
Collection: Journal Articles

Title Direct visualization of the structural transformation between the lyotropic liquid crystalline lamellar and bicontinuous cubic mesophase
Author(s) Tran, N
Zhai, J
Conn, C
Mulet, X
Waddington, L
Drummond, C
Year 2018
Journal name Journal of Physical Chemistry Letters
Volume number 9
Issue number 12
Start page 3397
End page 3402
Total pages 6
Publisher American Chemical Society
Abstract The transition between the lyotropic liquid crystalline lamellar and the bicontinuous cubic mesophase drives multiple fundamental cellular processes involving changes in cell membrane topology, including endocytosis and membrane budding. While several theoretical models have been proposed to explain this dynamic transformation, experimental validation of these models has been challenging because of the short-lived nature of the intermediates present during the phase transition. Herein, we report the direct observation of a lamellar-to-bicontinuous cubic phase transition in nanoscale dispersions using a combination of cryogenic transmission electron microscopy and static small-angle X-ray scattering. The results represent the first experimental confirmation of a theoretical model which proposed that the bicontinuous cubic phase originates from the center of a lamellar vesicle then propagates outward via the formation of interlamellar attachments and stalks. The observation was possible because of the precise control of the lipid composition to place the dispersion systems at the phase boundary of a lamellar and a cubic phase, allowing for the creation of long-lived structural intermediates. By the surveying of the nanoparticles using cryogenic transmission electron microscopy, a complete phase transition sequence was established.
Subject Nanochemistry and Supramolecular Chemistry
Colloid and Surface Chemistry
Nanomaterials
Keyword(s) x-ray
phase-transition
membrane-fusion
drug delivery
nanoparticles
monoolein
systems
lipids
DOI - identifier 10.1021/acs.jpclett.8b01110
Copyright notice © 2018 American Chemical Society
ISSN 1948-7185
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