Engineered Hydrogen-Bonded Glycopolymer Capsules and Their Interactions with Antigen Presenting Cells

Kempe, K, Xiang, S, Wilson, P, Rahim, M, Ju, Y, Whittaker, M, Haddleton, D, Plebanski, M, Caruso, F and Davis, T 2017, 'Engineered Hydrogen-Bonded Glycopolymer Capsules and Their Interactions with Antigen Presenting Cells', ACS Applied Materials and Interfaces, vol. 9, no. 7, pp. 6444-6452.


Document type: Journal Article
Collection: Journal Articles

Title Engineered Hydrogen-Bonded Glycopolymer Capsules and Their Interactions with Antigen Presenting Cells
Author(s) Kempe, K
Xiang, S
Wilson, P
Rahim, M
Ju, Y
Whittaker, M
Haddleton, D
Plebanski, M
Caruso, F
Davis, T
Year 2017
Journal name ACS Applied Materials and Interfaces
Volume number 9
Issue number 7
Start page 6444
End page 6452
Total pages 9
Publisher The American Chemical Society
Abstract Hollow glycopolymer microcapsules were fabricated by hydrogen-bonded layer-by-layer (LbL) assembly, and their interactions with a set of antigen presenting cells (APCs), including dendritic cells (DCs), macrophages (MACs), and myeloid derived suppressor cells (MDSCs), were investigated. The glycopolymers were obtained by cascade postpolymerization modifications of poly(oligo(2-ethyl-2-oxazoline methacrylate)-stat-glycidyl methacrylate) involving the modification of the glycidyl groups with propargylamine and the subsequent attachment of mannose azide by copper(I)-catalyzed azidealkyne cycloaddition (CuAAC). Multilayer assembly of the hydrogen-bonding pair (glycopolymer/poly(methacrylic acid) (PMA)) onto planar and particulate supports (SiO2 particles, d = 1.16 μm) yielded stable glycopolymer films upon cross-linking by CuAAC. The silica (SiO2) particle templates were removed yielding hollow monodisperse capsules, as demonstrated by fluorescence and scanning electron microscopy. Cellular uptake studies using flow cytometry revealed the preferential uptake of the capsules by DCs when compared to MACs or MDSCs. Mannosylated capsules showed a cytokine independent cis-upregulation of CD80 specifically on DCs and a trans-downregulation of PDL-1 on MDSCs. Thus, the glycopolymer capsules may have potential as vaccine carriers, as they are able to upregulate costimulatory molecules for immune cell stimulation on DCs and at the same time downregulate immune inhibitory receptors on suppressor APC such as MDSCs.
Subject Immunology not elsewhere classified
Keyword(s) CD80
Dendritic cells
Layer-by-layer
Macrophages
Mannose
MDSC
PDL-1
Poly(2-oxazoline)
DOI - identifier 10.1021/acsami.6b15459
Copyright notice © 2017 American Chemical Society
ISSN 1944-8244
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