Magnetic nanovectors for the development of DNA blood-stage malaria vaccines

Al-Deen, F, Xiang, S, Ma, C, Wilson, K, Coppel, R, Selomulya, C and Plebanski, M 2017, 'Magnetic nanovectors for the development of DNA blood-stage malaria vaccines', Nanomaterials, vol. 7, no. 2, 30, pp. 1-1.

Document type: Journal Article
Collection: Journal Articles

Title Magnetic nanovectors for the development of DNA blood-stage malaria vaccines
Author(s) Al-Deen, F
Xiang, S
Ma, C
Wilson, K
Coppel, R
Selomulya, C
Plebanski, M
Year 2017
Journal name Nanomaterials
Volume number 7
Issue number 2
Article Number 30
Start page 1
End page 1
Total pages 1
Publisher M D P I AG
Abstract DNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), and hyaluronic acid (HA) to deliver malaria DNA encoding Plasmodium yoelii (Py) merozoite surface protein MSP1(19) (SPIONs/PEI/DNA + HA gene complex) to dendritic cells and transfect them with high efficiency in vitro. Herein, we evaluate their immunogenicity in vivo by administering these potential vaccine complexes into BALB/c mice. The complexes induced antibodies against PyMSP1(19), with higher responses induced intraperitoneally than intramuscularly, and antibody levels further enhanced by applying an external magnetic field. The predominant IgG subclasses induced were IgG2a followed by IgG1 and IgG2b. The complexes further elicited high levels of interferon gamma (IFN-gamma), and moderate levels of interleukin (IL)-4 and IL-17 antigen-specific splenocytes, indicating induction of T helper 1 (Th1), Th2, and Th17 cell mediated immunity. The ability of such DNA/nanoparticle complexes to induce cytophilic antibodies together with broad spectrum cellular immunity may benefit malaria vaccines.
Subject Immunology not elsewhere classified
Keyword(s) Antibody
Hyaluronic acid
Immune response
Magnetic gene vector
Malaria DNA vaccine
Superparamagnetic iron oxide nanoparticles (SPIONs)
DOI - identifier 10.3390/nano7020030
Copyright notice © 2017 The Author(s)
ISSN 2079-4991
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