IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling

Li, S, Lefranc, M, Miles, J and Plebanski, M 2013, 'IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling', Nature Communications, vol. 4, 2333, pp. 1-1.


Document type: Journal Article
Collection: Journal Articles

Title IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling
Author(s) Li, S
Lefranc, M
Miles, J
Plebanski, M
Year 2013
Journal name Nature Communications
Volume number 4
Article Number 2333
Start page 1
End page 1
Total pages 1
Publisher Nature Publishing Group
Abstract T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 50 RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/ haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB.
Subject Immunology not elsewhere classified
DOI - identifier 10.1038/ncomms3333
Copyright notice © 2013 Macmillan Publishers Limited.
ISSN 2041-1723
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