Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves

Collins, D, Morahan, B, Garcia-Bustos, J, Doerig, C, Plebanski, M and Neild, A 2015, 'Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves', Nature Communications, vol. 6, 8686, pp. 1-1.


Document type: Journal Article
Collection: Journal Articles

Title Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves
Author(s) Collins, D
Morahan, B
Garcia-Bustos, J
Doerig, C
Plebanski, M
Neild, A
Year 2015
Journal name Nature Communications
Volume number 6
Article Number 8686
Start page 1
End page 1
Total pages 1
Publisher Nature Publishing Group
Abstract In single-cell analysis, cellular activity and parameters are assayed on an individual, rather than population-average basis. Essential to observing the activity of these cells over time is the ability to trap, pattern and retain them, for which previous single-cell-patterning work has principally made use of mechanical methods. While successful as a long-term cell-patterning strategy, these devices remain essentially single use. Here we introduce a new method for the patterning of multiple spatially separated single particles and cells using high-frequency acoustic fields with one cell per acoustic well. We characterize and demonstrate patterning for both a range of particle sizes and the capture and patterning of cells, including human lymphocytes and red blood cells infected by the malarial parasite Plasmodium falciparum. This ability is made possible by a hitherto unexplored regime where the acoustic wavelength is on the same order as the cell dimensions.
Subject Immunology not elsewhere classified
DOI - identifier 10.1038/ncomms9686
Copyright notice © Macmillan Publishers Limited.
ISSN 2041-1723
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