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Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery Al-Deen, F, Selomulya, C, Kong, Y, Xiang, S, Ma, C, Coppel, R and Plebanski, M 2014, 'Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery', Gene Therapy, vol. 21, no. 2, pp. 212-218. Document type: Journal Article Collection: Journal Articles Title Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery Author(s) Al-Deen, F Selomulya, C Kong, Y Xiang, S Ma, C Coppel, R Plebanski, M Year 2014 Journal name Gene Therapy Volume number 21 Issue number 2 Start page 212 End page 218 Total pages 7 Publisher Nature Publishing Group Abstract Dendritic cells (DC) targeting vaccines require high efficiency for uptake, followed by DC activation and maturation. We used magnetic vectors comprising polyethylenimine (PEI)-coated superparamagnetic iron oxide nanoparticles, with hyaluronic acid (HA) of different molecular weights (<10 and 900 kDa) to reduce cytotoxicity and to facilitate endocytosis of particles into DCs via specific surface receptors. DNA encoding Plasmodium yoelii merozoite surface protein 119 and a plasmid encoding yellow fluorescent gene were added to the magnetic complexes with various % charge ratios of HA: PEI. The presence of magnetic fields significantly enhanced DC transfection and maturation. Vectors containing a high-molecular-weight HA with 100% charge ratio of HA: PEI yielded a better transfection efficiency than others. This phenomenon was attributed to their longer molecular chains and higher mucoadhesive properties aiding DNA condensation and stability. Insights gained should improve the design of more effective DNA vaccine delivery systems. Subject Immunology not elsewhere classified Keyword(s) Dendritic cells DNA vaccine delivery Hyaluronic acid SPIONs DOI - identifier 10.1038/gt.2013.77 Copyright notice © 2014 Macmillan Publishers Limited. ISSN 0969-7128 Related Links Link Description https://dx.doi.org/10.1038/gt.2013.77 Link to Published Version   Versions Version Filter Type Mon, 10 Dec 2018, 13:38:02 EST Mon, 12 Aug 2019, 14:52:24 EST Filtered Full Citation counts:   Cited 18 times in Thomson Reuters Web of Science Article | Citations Cited 0 times in Scopus Article Altmetric details: Access Statistics: 8 Abstract Views  -  Detailed Statistics Created: Thu, 06 Dec 2018, 10:39:00 EST by Catalyst Administrator