Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery

Al-Deen, F, Selomulya, C, Kong, Y, Xiang, S, Ma, C, Coppel, R and Plebanski, M 2014, 'Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery', Gene Therapy, vol. 21, no. 2, pp. 212-218.


Document type: Journal Article
Collection: Journal Articles

Title Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery
Author(s) Al-Deen, F
Selomulya, C
Kong, Y
Xiang, S
Ma, C
Coppel, R
Plebanski, M
Year 2014
Journal name Gene Therapy
Volume number 21
Issue number 2
Start page 212
End page 218
Total pages 7
Publisher Nature Publishing Group
Abstract Dendritic cells (DC) targeting vaccines require high efficiency for uptake, followed by DC activation and maturation. We used magnetic vectors comprising polyethylenimine (PEI)-coated superparamagnetic iron oxide nanoparticles, with hyaluronic acid (HA) of different molecular weights (<10 and 900 kDa) to reduce cytotoxicity and to facilitate endocytosis of particles into DCs via specific surface receptors. DNA encoding Plasmodium yoelii merozoite surface protein 119 and a plasmid encoding yellow fluorescent gene were added to the magnetic complexes with various % charge ratios of HA: PEI. The presence of magnetic fields significantly enhanced DC transfection and maturation. Vectors containing a high-molecular-weight HA with 100% charge ratio of HA: PEI yielded a better transfection efficiency than others. This phenomenon was attributed to their longer molecular chains and higher mucoadhesive properties aiding DNA condensation and stability. Insights gained should improve the design of more effective DNA vaccine delivery systems.
Subject Immunology not elsewhere classified
Keyword(s) Dendritic cells
DNA vaccine delivery
Hyaluronic acid
SPIONs
DOI - identifier 10.1038/gt.2013.77
Copyright notice © 2014 Macmillan Publishers Limited.
ISSN 0969-7128
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 7 Abstract Views  -  Detailed Statistics
Created: Thu, 06 Dec 2018, 10:39:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us