On the efficacy of malaria DNA vaccination with magnetic gene vectors

Al-Deen, F, Ma, C, Xiang, S, Selomulya, C, Plebanski, M and Coppel, R 2013, 'On the efficacy of malaria DNA vaccination with magnetic gene vectors', Journal of Controlled Release, vol. 168, no. 1, pp. 10-17.

Document type: Journal Article
Collection: Journal Articles

Title On the efficacy of malaria DNA vaccination with magnetic gene vectors
Author(s) Al-Deen, F
Ma, C
Xiang, S
Selomulya, C
Plebanski, M
Coppel, R
Year 2013
Journal name Journal of Controlled Release
Volume number 168
Issue number 1
Start page 10
End page 17
Total pages 8
Publisher Elsevier BV
Abstract We investigated the efficacy and types of immune responses from plasmid malaria DNA vaccine encoding VR1020-PyMSP119condensed on the surface of polyethyleneimine (PEI)-coated SPIONs. In vivo mouse studies were done firstly to determine the optimum magnetic vector composition, and then to observe immune responses elicited when magnetic vectors were introduced via different administration routes. Higher serum antibody titers against PyMSP119were observed with intraperitoneal and intramuscular injections than subcutaneous and intradermal injections. Robust IgG2a and IgG1 responses were observed for intraperitoneal administration, which could be due to the physiology of peritoneum as a major reservoir of macrophages and dendritic cells. Heterologous DNA prime followed by single protein boost vaccination regime also enhanced IgG2a, IgG1, and IgG2b responses, indicating the induction of appropriate memory immunity that can be elicited by protein on recall. These outcomes support the possibility to design superparamagnetic nanoparticle-based DNA vaccines to optimally evoke desired antibody responses, useful for a variety of diseases including malaria.
Subject Immunology not elsewhere classified
Keyword(s) Administration routes
Antibody response
In vivo
Malaria DNA vaccine
DOI - identifier 10.1016/j.jconrel.2013.02.030
Copyright notice © 2013 Elsevier B.V. All rights reserved.
ISSN 0168-3659
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