Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor

Sanchez Huertas, C, Farina, D and Lechuga, L 2016, 'Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor', ACS Sensors, vol. 1, no. 6, pp. 748-756.


Document type: Journal Article
Collection: Journal Articles

Title Direct and Label-Free Quantification of Micro-RNA-181a at Attomolar Level in Complex Media Using a Nanophotonic Biosensor
Author(s) Sanchez Huertas, C
Farina, D
Lechuga, L
Year 2016
Journal name ACS Sensors
Volume number 1
Issue number 6
Start page 748
End page 756
Total pages 9
Publisher American Chemical Society
Abstract Micro-RNA signatures have emerged as advantageous biomarkers for disease prediction opening the route for the development of more direct and accurate therapies. There is an urgent need for reliable tools which can offer fast, highly sensitive, and selective detection of multiple miRNAs in complex matrices as opposed to the conventional techniques. Here, we demonstrate a nanophotonic biosensor with potential multiplexing capabilities based on interferometric bimodal nanowaveguides (BiMW) for ultrasensitive detection of microRNAs in complex media. Concretely, the BiMW biosensor has been employed for the detection and quantification of miR-181a at attomolar concentrations (LOD = 23 aM) directly, and for the first time, in human urine samples of bladder cancer patients with no need for prior sample purification or amplification steps. We demonstrate the extreme selectivity of our methodology for miR-181a detection showing the discrimination of homologous sequences at single nucleotide mismatch and its pre-miRNA. A significant overexpression of miR-181a in bladder cancer patients was appreciated when compared with healthy controls, suggesting the implication of this miRNA in bladder cancer. Our results show that the BiMW biosensor can be used as an ultrasensitive and specific diagnostic tool by the early and fast detection and quantification of microRNAs for the prediction of diseases (such as cancer) with well-defined microRNA signatures.
Subject Medical Devices
Medical Biotechnology Diagnostics (incl. Biosensors)
Keyword(s) biomarker
cancer
diagnosis
microRNA
miRNA
nanophotonic biosensor
waveguide interferometer
DOI - identifier 10.1021/acssensors.6b00162
Copyright notice © 2016 American Chemical Society.
ISSN 2379-3694
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