Sensitive and label-free detection of miRNA-145 by triplex formation

Avino, A, Sanchez Huertas, C, Lechuga, L and Eritja, R 2016, 'Sensitive and label-free detection of miRNA-145 by triplex formation', Analytical and Bioanalytical Chemistry, vol. 408, no. 3, pp. 885-893.


Document type: Journal Article
Collection: Journal Articles

Title Sensitive and label-free detection of miRNA-145 by triplex formation
Author(s) Avino, A
Sanchez Huertas, C
Lechuga, L
Eritja, R
Year 2016
Journal name Analytical and Bioanalytical Chemistry
Volume number 408
Issue number 3
Start page 885
End page 893
Total pages 9
Publisher Springer
Abstract The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
Subject Medical Biotechnology Diagnostics (incl. Biosensors)
Keyword(s) Triplex
Target miRNA-145
SPR biosensor
Parallel tail-clamp
8-Amino-2′-deoxyguanosine
DOI - identifier 10.1007/s00216-015-9180-6
Copyright notice © 2015 Springer-Verlag Berlin Heidelberg.
ISSN 1618-2642
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