Detection, Labeling, and Culture of Lung Stem and Progenitor Cells

Bertoncello, I, Carraro, G and McQualter, J 2018, 'Detection, Labeling, and Culture of Lung Stem and Progenitor Cells' in Shree Ram Singh and Pranela Rameshwar (ed.) Somatic Stem Cells, Springer Science+Business Media, LLC, New York, United States, pp. 167-181.


Document type: Book Chapter
Collection: Book Chapters

Title Detection, Labeling, and Culture of Lung Stem and Progenitor Cells
Author(s) Bertoncello, I
Carraro, G
McQualter, J
Year 2018
Title of book Somatic Stem Cells
Publisher Springer Science+Business Media, LLC
Place of publication New York, United States
Editor(s) Shree Ram Singh and Pranela Rameshwar
Start page 167
End page 181
Subjects Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
Summary Identification, isolation, and clonal culture of stem cells is essential for understanding their proliferative and differentiation potential, and the cellular and molecular mechanisms that regulate their fate. Akin to development in vivo, the in vitro growth of adult lung epithelial stem cells requires support of mesenchymal-derived growth factors. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45neg CD31neg EpCAMpos CD104pos CD24low, and mesenchymal cells are defined by the phenotype CD45neg CD31neg EpCAMneg Sca-1hi. Here we describe a method for primary cell isolation from the adult mouse lung, a flow cytometry strategy for fractionation of epithelial stem/progenitor cells and mesenchymal cells, and a three-dimensional epithelial colony-forming assay.
Copyright notice © Springer Science+Business Media, LLC, part of Springer Nature 2018
Keyword(s) Lung epithelium
Stem cells
Colony-forming assay
Flow cytometry
DOI - identifier 10.1007/978-1-4939-8697-2_11
ISBN 9781493986965
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