Low-Fouling and Biodegradable Protein-Based Particles for Thrombus Imaging

Bonnard, T, Jayapadman, A, Azzahra Putri, J and Angelovich, T.A. et al, 2018, 'Low-Fouling and Biodegradable Protein-Based Particles for Thrombus Imaging', ACS Nano, vol. 12, no. 7, pp. 6988-6996.

Document type: Journal Article
Collection: Journal Articles

Title Low-Fouling and Biodegradable Protein-Based Particles for Thrombus Imaging
Author(s) Bonnard, T
Jayapadman, A
Azzahra Putri, J
Angelovich, T.A. et al,
Year 2018
Journal name ACS Nano
Volume number 12
Issue number 7
Start page 6988
End page 6996
Total pages 9
Publisher American Chemical Society
Abstract Nanomedicine holds great promise for vascular disease diagnosis and specific therapy, yet rapid sequestration by the mononuclear phagocytic system limits the efficacy of particle-based agents. The use of low-fouling polymers, such as poly(ethylene glycol), efficiently reduces this immune recognition, but these nondegradable polymers can accumulate in the human body and may cause adverse effects after prolonged use. Thus, new particle formulations combining stealth, low immunogenicity and biocompatible features are required to enable clinical use. Here, a low-fouling particle platform is described using exclusively protein material. A recombinant protein with superior hydrophilic characteristics provided by the amino acid repeat proline, alanine, and serine (PAS) is designed and cross-linked into particles with lysine (K) and polyglutamic acid (E) using mesoporous silica templating. The obtained PASKE particles have low-fouling behavior, have a prolonged circulation time compared to albumin-based particles, and are rapidly degraded in the cell's lysosomal compartment. When labeled with near-infrared fluorescent molecules and functionalized with an anti-glycoprotein IIb/IIIa single-chain antibody targeting activated platelets, the particles show potential as a noninvasive molecular imaging tool in a mouse model of carotid artery thrombosis. The PASKE particles constitute a promising biodegradable and versatile platform for molecular imaging of vascular diseases.
Subject Nanobiotechnology
Cardiology (incl. Cardiovascular Diseases)
Keyword(s) biodegradability
near-infrared imaging
protein particles
DOI - identifier 10.1021/acsnano.8b02588
Copyright notice © 2018 American Chemical Society
ISSN 1936-0851
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