Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes

Khorramdelazad, M, Bar, I, Whatmore, P, Smetham, G, Bhaskarla, V, Yang, Y, Bai, S, Mantri, N, Zhou, Y and Ford, R 2018, 'Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes', BMC Genomics, vol. 19, no. 1, pp. 1-21.


Document type: Journal Article
Collection: Journal Articles

Title Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes
Author(s) Khorramdelazad, M
Bar, I
Whatmore, P
Smetham, G
Bhaskarla, V
Yang, Y
Bai, S
Mantri, N
Zhou, Y
Ford, R
Year 2018
Journal name BMC Genomics
Volume number 19
Issue number 1
Start page 1
End page 21
Total pages 21
Publisher BioMed Central Ltd.
Abstract Background: Ascochyta blight, caused by the fungus Ascochyta lentis, is one of the most destructive lentil diseases worldwide, resulting in over $16 million AUD annual loss in Australia alone. The use of resistant cultivars is currently considered the most effective and environmentally sustainable strategy to control this disease. However, little is known about the genes and molecular mechanisms underlying lentil resistance against A. lentis. Results: To uncover the genetic basis of lentil resistance to A. lentis, differentially expressed genes were profiled in lentil plants during the early stages of A. lentis infection. The resistant 'ILL7537' and susceptible 'ILL6002' lentil genotypes were examined at 2, 6, and 24 h post inoculation utilising high throughput RNA-Sequencing. Genotype and time-dependent differential expression analysis identified genes which play key roles in several functions of the defence response: fungal elicitors recognition and early signalling; structural response; biochemical response; transcription regulators; hypersensitive reaction and cell death; and systemic acquired resistance. Overall, the resistant genotype displayed an earlier and faster detection and signalling response to the A. lentis infection and demonstrated higher expression levels of structural defence-related genes. Conclusions: This study presents a first-time defence-related transcriptome of lentil to A. lentis, including a comprehensive characterisation of the molecular mechanism through which defence against A. lentis is induced in the resistant lentil genotype.
Subject Gene Expression (incl. Microarray and other genome-wide approaches)
Crop and Pasture Protection (Pests, Diseases and Weeds)
Keyword(s) Ascochyta lentis
De novo assembly
Defence response
Fabaceae
Lens culinaris
Lentil
RNA sequencing and transcriptome analysis
DOI - identifier 10.1186/s12864-018-4488-1
Copyright notice © The Author(s) 2018. Creative Commons Attribution 4.0 International License
ISSN 1471-2164
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Altmetric details:
Access Statistics: 13 Abstract Views  -  Detailed Statistics
Created: Fri, 14 Dec 2018, 16:06:00 EST by Catalyst Administrator
© 2014 RMIT Research Repository • Powered by Fez SoftwareContact us