Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor

Chan, K, Ye, W, Zhang, Y, Xiao, L, Leung, P, Li, Y and Yang, M 2013, 'Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor', Biosensors and Bioelectronics, vol. 41, no. 1, pp. 532-537.


Document type: Journal Article
Collection: Journal Articles

Title Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor
Author(s) Chan, K
Ye, W
Zhang, Y
Xiao, L
Leung, P
Li, Y
Yang, M
Year 2013
Journal name Biosensors and Bioelectronics
Volume number 41
Issue number 1
Start page 532
End page 537
Total pages 6
Publisher Elsevier
Abstract In this paper, biofunctional magnetic beads were investigated for bacterial cells concentration in a nanoporous alumina membrane based immunosensor for ultra-sensitive detection of E. coli O157:H7. The specific antibody modified magnetic beads were used for concentration of E coli O157:H7 from samples in a small region to enhance sensitivity. The magnetic bead conjugated E. coli O157:H7 cells were then captured on the nanoporous alumina membrane with immobilized antibody via assembled PEG-silane linker. Scanning electron microscopy and fluorescent microscopy were used to demonstrate the magnetic bead-bacteria cell conjugation and bacteria cells magnetic concentration, respectively. Impedance spectroscopy was used to monitor the pure E coli O157:H7 cells and magnetic bead conjugated E coli O157:H7 cells binding on antibody immobilized nanoporous membrane with or without magnetic field. Compared with direct detection of pure bacteria cells, this method via magnetic bead conjugation and concentration demonstrated the ultrasensitivity of 10. CFU/mL for E coli O157:H7 detection.
Subject Biomedical Instrumentation
Keyword(s) Bacteria detection
Impedance sensor
Magnetic bead concentration
Nanoporous membrane
DOI - identifier 10.1016/j.bios.2012.09.016
Copyright notice © 2012 Elsevier B.V. All rights reserved.
ISSN 0956-5663
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