A hydrophilic polymer based microfluidic system with planar patch clamp electrode array for electrophysiological measurement from cells

Xu, B, Ye, W, Zhang, Y, Shi, J, Chan, C, Yao, X and Yang, M 2014, 'A hydrophilic polymer based microfluidic system with planar patch clamp electrode array for electrophysiological measurement from cells', Biosensors and Bioelectronics, vol. 53, pp. 187-192.


Document type: Journal Article
Collection: Journal Articles

Title A hydrophilic polymer based microfluidic system with planar patch clamp electrode array for electrophysiological measurement from cells
Author(s) Xu, B
Ye, W
Zhang, Y
Shi, J
Chan, C
Yao, X
Yang, M
Year 2014
Journal name Biosensors and Bioelectronics
Volume number 53
Start page 187
End page 192
Total pages 6
Publisher Elsevier
Abstract This paper presents a microfluidic planar patch clamp system based on a hydrophilic polymer poly(ethylene glycol) diacrylate (PEGDA) for whole cell current recording. The whole chip is fabricated by UV-assisted molding method for both microfluidic channel structure and planar electrode partition. This hydrophilic patch clamp chip has demonstrated a relatively high gigaseal success rate of 44% without surface modification compared with PDMS based patch clamp devices. This chip also shows a capability of rapid intracellular and extracellular solution exchange with high stability of gigaseals. The capillary flow kinetic experiments demonstrate that the flow rates of PEGDA microfluidic channels are around two orders of magnitude greater than those for PDMS-glass channels with the same channel dimensions. This hydrophilic polymer based patch clamp chips have significant advantages over current PDMS elastomer based systems such as no need for surface modification, much higher success rate of cell gigaseals and rapid solution exchange with stable cell gigaseals. Our results indicate the potential of these devices to serve as useful tools for pharmaceutical screening and biosensing tasks. © 2013 Elsevier B.V.
Subject Biomedical Instrumentation
Keyword(s) Biosensing
Hydrophilic polymer
Patch clamp
Rapid solution exchange
DOI - identifier 10.1016/j.bios.2013.09.049
Copyright notice © 2013 Elsevier B.V. All rights reserved.
ISSN 0956-5663
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