Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study

Liu, Z, Xiao, L, Xu, B, Zhang, Y, Mak, A, Li, Y, Man, W and Yang, M 2012, 'Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study', Biomicrofluidics, vol. 6, no. 2, pp. 1-13.


Document type: Journal Article
Collection: Journal Articles

Title Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study
Author(s) Liu, Z
Xiao, L
Xu, B
Zhang, Y
Mak, A
Li, Y
Man, W
Yang, M
Year 2012
Journal name Biomicrofluidics
Volume number 6
Issue number 2
Start page 1
End page 13
Total pages 13
Publisher A I P Publishing
Abstract Precisely controlling the spatial distribution of biomolecules on biomaterial surface is important for directing cellular activities in the controlled cell microenvironment. This paper describes a polydimethylsiloxane (PDMS) gradient-generating microfluidic device to immobilize the gradient of cellular adhesive Arg-Gly-Asp (RGD) peptide on poly (ethylene glycol) (PEG) hydrogel. Hydrogels are formed by exposing the mixture of PEG diacrylate (PEGDA), acryloyl-PEG-RGD, and photo-initiator with ultraviolet light. The microfluidic chip was simulated by a fluid dynamic model for the biomolecule diffusion process and gradient generation. PEG hydrogel covalently immobilized with RGD peptide gradient was fabricated in this microfluidic device by photo-polymerization. Bone marrow derived rat mesenchymal stem cells (MSCs) were then cultured on the surface of RGD gradient PEG hydrogel. Cell adhesion of rat MSCs on PEG hydrogel with various RGD gradients were then qualitatively and quantitatively analyzed by immunostaining method. MSCs cultured on PEG hydrogel surface with RGD gradient showed a grated fashion for cell adhesion and spreading that was proportional to RGD concentration. It was also found that 0.107-0.143 mM was the critical RGD concentration range for MSCs maximum adhesion on PEG hydrogel.
Subject Biomedical Instrumentation
DOI - identifier 10.1063/1.4704522
Copyright notice © 2012 American Institute of Physics
ISSN 1932-1058
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