Imaging single cells in a beam of live cyanobacteria with an X-ray laser

van der Schot, G, Svenda, M, Maia, F and Martin, A 2015, 'Imaging single cells in a beam of live cyanobacteria with an X-ray laser', Nature Communications, vol. 6, 5704, pp. 1-9.


Document type: Journal Article
Collection: Journal Articles

Title Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Author(s) van der Schot, G
Svenda, M
Maia, F
Martin, A
Year 2015
Journal name Nature Communications
Volume number 6
Article Number 5704
Start page 1
End page 9
Total pages 9
Publisher Nature Publishing Group
Abstract All rights reserved. There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.
Subject Condensed Matter Imaging
DOI - identifier 10.1038/ncomms6704
Copyright notice © 2014 Macmillan Publishers Limited.
ISSN 2041-1723
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