Single-particle structure determination by correlations of snapshot X-ray diffraction patterns

Starodub, D, Aquila, A, Sasa Bajt, Barthelmess, M and Martin, A 2012, 'Single-particle structure determination by correlations of snapshot X-ray diffraction patterns', Nature Communications, vol. 3, 1276, pp. 1-7.

Document type: Journal Article
Collection: Journal Articles

Title Single-particle structure determination by correlations of snapshot X-ray diffraction patterns
Author(s) Starodub, D
Aquila, A
Sasa Bajt
Barthelmess, M
Martin, A
Year 2012
Journal name Nature Communications
Volume number 3
Article Number 1276
Start page 1
End page 7
Total pages 7
Publisher Macmillan Publishers Limited.
Abstract Diffractive imaging with free-electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra-short, ultra-bright X-ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three-dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three-dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single-particle femtosecond X-ray diffraction patterns of nearly identical samples in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self-assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution.
Subject Condensed Matter Imaging
DOI - identifier 10.1038/ncomms2288
Copyright notice © 2012 Macmillan Publishers Limited.
ISSN 2041-1723
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