Development of silk fibroin modified poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) nanoparticles in supercritical CO2

Zhao, Z, Li, Y, Zhang, Y, Chen, A, Li, G, Zhang, J and Xie, M 2014, 'Development of silk fibroin modified poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) nanoparticles in supercritical CO2', Powder Technology, vol. 268, pp. 118-125.


Document type: Journal Article
Collection: Journal Articles

Title Development of silk fibroin modified poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) nanoparticles in supercritical CO2
Author(s) Zhao, Z
Li, Y
Zhang, Y
Chen, A
Li, G
Zhang, J
Xie, M
Year 2014
Journal name Powder Technology
Volume number 268
Start page 118
End page 125
Total pages 8
Publisher Elsevier
Abstract Silk fibroin (SF) modified poly(l-lactide)poly(ethylene glycol)poly(l-lactide) (SF/PLLAPEGPLLA) nanoparticles were successfully fabricated in a process of solution-enhanced dispersion by supercritical CO2 (SEDS). The SF/PLLAPEGPLLA nanoparticles exhibited a composite structure with mean particle size of 634 nm and silk fibroin wrapped with PLLAPEGPLLA triblock polymer. Fourier transform infrared spectroscopy (FTIR) measurement indicated that minor secondary structural changes of silk fibroin occurred after the SEDS process. X-ray powder diffraction (XRPD) analysis supported the results of FTIR measurement and also revealed that the SEDS process resulted in a notable decrease in crystallinity of the PLLAPEGPLLA. In vitro cytotoxicity evaluation by MTS assay indicated that SF/PLLAPEGPLLA nanoparticles exhibited better biocompatibility than PLLAPEGPLLA nanoparticles. Fluorescence microscopy observation and flow cytometric analysis suggested that SF/PLLAPEGPLLA nanoparticles could be internalized into fibroblasts in a time-dependent manner and also possessed faster cell adhesion and internalization ability than PLLAPEGPLLA nanoparticles. In conclusion, SF/PLLAPEGPLLA nanoparticles prepared by the SEDS process could be used as potential biomaterials in the biomedical field, especially nanoparticle drug delivery systems.
Subject Biomaterials
Keyword(s) Biocompatibility
Cellular uptake
Nanoparticles
PLLA-PEG-PLLA
Silk fibroin
Supercritical CO 2
DOI - identifier 10.1016/j.powtec.2014.07.029
Copyright notice © 2014 Elsevier B.V. All rights reserved.
ISSN 0032-5910
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